Multi-organ functions of yolk sac during human early development

Multi-organ functions of yolk sac during human early development
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DOI：
10.1101/2022.08.03.502475
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发表时间：
2022-08
期刊：
bioRxiv
影响因子：
--
通讯作者：
R. Botting;Issac Goh;Antony Rose;S. Webb;J. Engelbert;Y. Gitton;Emily Stephenson;Mariana Quiroga Londoño;M. Mather;N. Mende;Ivan Imaz-Rosshandler;Dave Horsfall;Daniela Basurto-Lozada;Nana-Jane Chipampe;Victoria Rook;P. Mazin;M. Vijayabaskar;R. Hannah;L. Gambardella;Kile Green;S. Ballereau;Megumi Inoue;L. Tuck;Valentina Lorenzi;Kwasi Kwakwa;Clara Alsinet;B. Olabi;M. Miah;C. Admane;Dorin-Mirel Popescu;M. Acres;D. Dixon;R. Coulthard;S. Lisgo;D. Henderson;Emma Dann;Chenqu Suo;S. Kinston;Jong-Eun Park;K. Polański;Stijn van Dongen;K. Meyer;M. D. de Bruijn;J. Palis;S. Behjati;E. Laurenti;Nicola K. Wilson;R. Vento-Tormo;A. Chédotal;O. Bayraktar;I. Roberts;Laura Jardine;B. Göttgens;Sarah A. Teichmann;M. Haniffa
R. Botting;Issac Goh;Antony Rose;S. Webb;J. Engelbert;Y. Gitton;Emily Stephenson;Mariana Quiroga Londoño;M. Mather;N. Mende;Ivan Imaz-Rosshandler;Dave Horsfall;Daniela Basurto-Lozada;Nana-Jane Chipampe;Victoria Rook;P. Mazin;M. Vijayabaskar;R. Hannah;L. Gambardella;Kile Green;S. Ballereau;Megumi Inoue;L. Tuck;Valentina Lorenzi;Kwasi Kwakwa;Clara Alsinet;B. Olabi;M. Miah;C. Admane;Dorin-Mirel Popescu;M. Acres;D. Dixon;R. Coulthard;S. Lisgo;D. Henderson;Emma Dann;Chenqu Suo;S. Kinston;Jong-Eun Park;K. Polański;Stijn van Dongen;K. Meyer;M. D. de Bruijn;J. Palis;S. Behjati;E. Laurenti;Nicola K. Wilson;R. Vento-Tormo;A. Chédotal;O. Bayraktar;I. Roberts;Laura Jardine;B. Göttgens;Sarah A. Teichmann;M. Haniffa
中科院分区：
其他
文献类型：
--
作者：
R. Botting;Issac Goh;Antony Rose;S. Webb;J. Engelbert;Y. Gitton;Emily Stephenson;Mariana Quiroga Londoño;M. Mather;N. Mende;Ivan Imaz-Rosshandler;Dave Horsfall;Daniela Basurto-Lozada;Nana-Jane Chipampe;Victoria Rook;P. Mazin;M. Vijayabaskar;R. Hannah;L. Gambardella;Kile Green;S. Ballereau;Megumi Inoue;L. Tuck;Valentina Lorenzi;Kwasi Kwakwa;Clara Alsinet;B. Olabi;M. Miah;C. Admane;Dorin-Mirel Popescu;M. Acres;D. Dixon;R. Coulthard;S. Lisgo;D. Henderson;Emma Dann;Chenqu Suo;S. Kinston;Jong-Eun Park;K. Polański;Stijn van Dongen;K. Meyer;M. D. de Bruijn;J. Palis;S. Behjati;E. Laurenti;Nicola K. Wilson;R. Vento-Tormo;A. Chédotal;O. Bayraktar;I. Roberts;Laura Jardine;B. Göttgens;Sarah A. Teichmann;M. Haniffa

文献摘要

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卵黄囊（YS）代表了一种进化保守的胚胎外结构，确保及时向发育中的胚胎提供营养支持和氧气。然而，YS在人类中的定义仍然不明确。因此，通过整合多组蛋白和基因表达数据，我们组装了一个完整的人类YS单细胞3D图谱，时间为受孕后3-8周。我们揭示了YS是一个原始和最终的造血位点，包括YS特异性加速巨噬细胞产生的途径，营养/代谢支持的来源和氧携带能力的调节剂。我们重建了YS造血内皮中原始造血干细胞和祖细胞的出现，以及它们在基质支持调节下的衰退，因为胚胎内器官专门承担这些功能。因此，YS的功能是“三合一”，揭示了怀孕过程中重要机体功能的多方面接力。卵黄囊是人类妊娠过程中重要机体功能接力的重要中转站。
The yolk sac (YS) represents an evolutionarily-conserved extraembryonic structure that ensures timely delivery of nutritional support and oxygen to the developing embryo. However, the YS remains ill-defined in humans. We therefore assemble a complete single cell 3D map of human YS from 3-8 post conception weeks by integrating multiomic protein and gene expression data. We reveal the YS as a site of primitive and definitive haematopoiesis including a YS-specific accelerated route to macrophage production, a source of nutritional/metabolic support and a regulator of oxygen-carrying capacity. We reconstruct the emergence of primitive haematopoietic stem and progenitor cells from YS hemogenic endothelium and their decline upon stromal support modulation as intraembryonic organs specialise to assume these functions. The YS therefore functions as ‘three organs in one’ revealing a multifaceted relay of vital organismal functions as pregnancy proceeds. One Sentence Summary Human yolk sac is a key staging post in a relay of vital organismal functions during human pregnancy.