Identification of major proteins in maize egg cells
Identification of major proteins in maize egg cells
复制标题
DOI：
10.1093/pcp/pch161
复制
发表时间：
2004-10-01
影响因子：
4.9
通讯作者：
Kranz, E
中科院分区：
文献类型：
--
作者：
Okamoto, T;Higuchi, K;Kranz, E
在大多数开花植物中，雌配子体在深深嵌入子房的胚珠中发育。通过双受精，卵细胞与精子细胞融合，产生受精卵，受精卵发育成胚胎。在本研究中，我们利用聚丙烯酰胺凝胶电泳法和随后的基于质谱仪的蛋白质组学技术对卵细胞裂解产物进行了分析，并鉴定了卵细胞中表达的主要蛋白质组分。已鉴定的蛋白质包括3-磷酸甘油醛脱氢酶、3-磷酸甘油酸激酶和磷酸丙糖异构酶三种胞浆酶，两种线粒体蛋白，三磷酸腺苷合成酶β亚基和腺嘌呤核苷酸转运体，以及膜联蛋白p35。此外，将这些蛋白在卵细胞中的表达水平与早期胚胎、中央细胞和悬浮细胞中的表达水平进行了比较。Annexin p35仅在卵细胞中高表达，3-磷酸甘油醛脱氢酶、3-磷酸甘油酸激酶和腺苷核苷酸转运蛋白在卵细胞中的表达水平高于中央细胞和培养细胞。这些结果表明，卵细胞和受精卵中的Annexin p35参与了胞壁物质的胞吐作用，这种胞吐作用是由受精引起的胞内钙水平的增加引起的，并且卵细胞中含有丰富的能量代谢酶亚群。
In most flowering plants, the female gametophyte develops in an ovule deeply embedded in the ovary. Through double fertilization, the egg cell fuses with the sperm cell, resulting in a zygote, which develops into the embryo. In the present study, we analyzed egg cell lysates by polyacrylamide gel electrophoresis and subsequent mass spectrometry-based proteomics technology, and identified major protein components expressed in the egg cell. The identified proteins included three cytosolic enzymes of the glycolytic pathway, glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase and triosephosphate isomerase, two mitochondrial proteins, the ATP synthase beta-subunit and an adenine nucleotide transporter, and annexin p35. In addition, expression levels of these proteins in the egg cell were compared with those in the early embryo, the central cell and the suspension cell. Annexin p35 was highly expressed only in the egg cell, and glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase and the adenine nucleotide transporter were expressed at higher levels in egg cells than in central and cultured cells. These results indicate that annexin p35 in the egg cell and zygote is involved in the exocytosis of cell wall materials, which is induced by a fertilization-triggered increase in cytosolic Ca2+ levels, and that the egg cell is rich in an enzyme subset for the energy metabolism.