Functionality of CD4+ and CD8+ T cells from tonsillar tissue
Functionality of CD4+ and CD8+ T cells from tonsillar tissue
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DOI：
10.1111/j.1365-2249.2012.04573.x
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发表时间：
2012-05-01
影响因子：
4.6
通讯作者：
Torre-Bouscoulet, L.
中科院分区：
文献类型：
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作者：
Sada-Ovalle, I.;Talayero, A.;Torre-Bouscoulet, L.
多年来，扁桃体切除术一直是儿童治疗慢性或复发性急性扁桃体炎的常规方法。扁桃体是次级淋巴器官，是保护消化和呼吸道免受潜在入侵微生物侵袭的主要屏障。它们被用作淋巴组织的来源；然而，尽管有数百篇关于扁桃体切除术的论文发表，但还没有关于慢性感染扁桃体的CD4+和CD8+T细胞功能的研究发表。这项研究的目的是分析相对于扁桃体组织的CD4+和CD8+T细胞的功能。我们使用一种负担得起的方法来测量抗原特异性CD4+T细胞的频率、CD8+T细胞的直接体外细胞毒作用、记忆性T细胞表型、细胞因子谱和DC表型。我们的结果表明，扁桃体组织中的CD4+和CD8+T细胞具有完全的功能，如产生细胞因子、脱颗粒和分化为效应记忆T细胞的能力。
For many years, tonsillectomy has been used routinely in children to treat chronic or recurrent acute tonsillitis. Palatine tonsils are secondary lymphoid organs and the major barrier protecting the digestive and respiratory tracts from potential invasive microorganisms. They have been used as sources of lymphoid tissue; however, despite the hundreds of papers published on tonsillectomy, no studies addressing the functionality of the CD4+ and CD8+ T cells from chronically infected tonsils have yet been published. The aim of this study was to analyse the functionality of the CD4+ and CD8+ T cells with respect to tonsillar tissue. We used an affordable approach to measure the frequency of antigen-specific CD4+ T cells, the direct ex-vivo cytotoxicity of CD8+ T cells, memory T cell phenotype, cytokine profile and DC phenotype. Our results demonstrate that CD4+ and CD8+ T cells from tonsillar tissue are totally functional, as shown by their ability to produce cytokines, to degranulate and to differentiate into effector-memory T cells.