Enhancement of monocyte migration and phagocytosis by the bovine immunodeficiency-like virus Gag proteins.
Enhancement of monocyte migration and phagocytosis by the bovine immunodeficiency-like virus Gag proteins.
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牛免疫缺陷样病毒 Gag 蛋白增强单核细胞迁移和吞噬作用。
DOI：
10.1097/00042560-199701010-00004
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Roth,JA
中科院分区：
文献类型：
--
作者：
Rovid,AH;Roth,JA
牛免疫缺陷样病毒（BIV）感染细胞的上清液先前已被证明影响单核细胞的随机迁移，趋化性，吞噬作用和抗体依赖性细胞介导的细胞毒性（ADCC）在体外。本报告中的实验表明，BIV Gag（核心）蛋白可以增强单核细胞的随机迁移，趋化性和吞噬作用。来自BIV感染细胞的上清液含有10-30和30-50 kDa蛋白，其显著（p< 0.05）增加单核细胞趋化性。30-50 kDa的蛋白质可以通过有限的蛋白水解被切割成10-30 kDa的活性组分。亲和纯化与单克隆抗p26（衣壳）抗体产生的制剂是积极的随机迁移，趋化性，和吞噬试验，但不影响ADCC。此外，亲和纯化的制剂的活性可以特异性中和的超免疫兔血清对BIV Gag蛋白。主要由BIV p26组成的重组Gag蛋白也增强了单核细胞的随机和趋化性迁移。因此，似乎用亲和纯化的BIV Gag蛋白或重组Gag蛋白直接处理能够显著影响体外正常单核细胞的功能。影响单核细胞迁移和吞噬作用的因素似乎是BIV Gag前体的一种或多种分解产物，特别是含有p26（衣壳）蛋白的那些。
Supernatants from bovine immunodeficiency-like virus (BIV)-infected cells have been previously shown to affect monocyte random migration, chemotaxis, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC) in vitro. The experiments in this report demonstrate that the BIV Gag (core) proteins can enhance monocyte random migration, chemotaxis, and phagocytosis. Supernatants from BIV-infected cells contained 10-30 and 30-50 kDa proteins, which significantly (p< 0.05) increased monocyte chemotaxis. The 30-50 kDa protein (s) could be cleaved by limited proteolysis into 10-30 kDa active components. Affinity purification with monoclonal anti-p26 (capsid) antibodies yielded preparations that were active in the random migration, chemotaxis, and phagocytosis assays, but did not affect ADCC. Furthermore, activity of the affinity purified preparation could be specifically neutralized by hyperimmune rabbit serum against BIV Gag proteins. A recombinant Gag protein, consisting primarily of BIV p26, also enhanced monocyte random and chemotactic migration. It appears, therefore, that direct treatment with affinity-purified BIV Gag proteins or a recombinant Gag protein, is able to significantly affect the function of normal monocytes in vitro. Factors affecting monocyte migration and phagocytosis appear to be one or more breakdown products of the BIV Gag precursor, particularly those containing the p26 (capsid) protein.