Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair
Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair
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DOI：
10.1016/s0006-8993(03)02539-3
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发表时间：
2003-06-06
期刊：
影响因子：
2.9
通讯作者：
Ochi, M
中科院分区：
文献类型：
--
作者：
Murakami, T;Fujimoto, Y;Ochi, M
在大鼠坐骨神经缺损中进行了胶原凝胶包埋神经祖细胞再生周围神经的基础实验。首先，将来源于胎鼠海马的神经祖细胞置于含间胶原的培养基中培养，8天后获得抗巢蛋白抗体阳性的神经球。这些细胞分化为抗胶质原纤维酸性蛋白（GFAP）抗体阳性的星形胶质细胞、抗半乳糖脑苷（GalC）抗体阳性的少突胶质细胞和抗神经丝200 （NF200）抗体阳性的神经元，并具有延伸轴突的能力。它们也分化为抗s100和抗p75抗体阳性的雪旺样支持细胞。接下来，在成熟大鼠坐骨神经上制备一个15mm的缺损，并用胶原凝胶包埋的充满神经元祖细胞（I X 105）的硅胶管桥接神经。移植后的神经元祖细胞提前用5-溴-2-脱氧尿苷（BrdU）标记。移植后6周和10周观察再生组织时，髓鞘纤维的数量和直径均比不含神经元祖细胞的对照管明显增加。在再生神经中检测动作电位。在再生组织中也观察到抗brdu抗体和抗s100或抗p75抗体均阳性的细胞，认为部分移植物神经干细胞已分化为雪旺细胞样支持细胞。从这些结果来看，来源于胎鼠海马的神经祖细胞在胶原凝胶中保留了其增殖和分化能力，当移植到周围神经缺损部位时，部分神经元祖细胞分化为支持细胞，有助于促进轴突再生。(C) 2003 Elsevier Science B.V.版权所有
A basic experiment of peripheral nerve regeneration using neuronal progenitor cells embedded in collagen gel was performed in a rat sciatic nerve defect. First, when neuronal progenitor cells derived from the fetal rat hippocampus were cultured in atelocollagen-containing medium, neurospheres positive for anti-nestin antibody were confirmed after 8 days. These cells differentiated into astrocytes positive for anti-glial fibrillary acidic protein (GFAP) antibody, oligodendrocytes positive for anti-galactocerebroside (GalC) antibody and neurons positive for anti-neurofilament 200 (NF200) antibody, and they were capable of extending axons. They also differentiated into Schwann-like supportive cells positive for anti-s100 and anti-p75 antibody. Next, a 15-mm defect was prepared in the sciatic nerve of mature rats, and the nerve was bridged with a silicone tube filled with neuronal progenitor cells (I X 105) embedded in collagen gel. The transplanted neuronal progenitor cells were labeled in advance with 5-bromo-2-deoxyuridine (BrdU). When the regenerated tissue was examined 6 weeks and 10 weeks after grafting, the number and diameter of myelinated fibers were significantly increased compared with a control tube without neuronal progenitor cells. Action potentials were detected in the regenerated nerve. Also, cells positive for both anti-BrdU antibody and anti-S100 or anti-p75 antibody were observed in the regenerated tissue, and part of the grafted neural stem cells were considered to have differentiated into Schwann cell-like supportive cells. From these results neuronal progenitor cells derived from the fetal rat hippocampus are considered to retain their proliferative and differentiating abilities in collagen gel, and when transplanted to a site of peripheral nerve defect, part of them differentiate into supportive cells and they contributed to promotion of axonal regeneration. (C) 2003 Elsevier Science B.V. All rights reserved.