Phylogenetic relationships among ascomycetous truffles and the true and false morels inferred from 18S and 28S ribosomal DNA sequence analysis
Phylogenetic relationships among ascomycetous truffles and the true and false morels inferred from 18S and 28S ribosomal DNA sequence analysis
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DOI：
10.1080/00275514.1997.12026754
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发表时间：
1997
期刊：
影响因子：
2.8
通讯作者：
K. O’Donnell;E. Cigelnik;N. S. Weber;J. Trappe
中科院分区：
文献类型：
--
作者：
K. O’Donnell;E. Cigelnik;N. S. Weber;J. Trappe
利用两个核编码核糖体DNA基因的序列，对子囊菌块菌与真羊肚菌和假羊肚菌的系统发育关系进行了研究。数据包括29个分类群的18 SrDNA和部分28 SrDNA序列。采用最大简约法（MP）、邻接法（NJ）和最大似然法（ML）对个体和合并数据集进行分析。对包含3个已发表的18 S序列和2358个核苷酸特征的组合数据集进行简约分析，得到一个1728步的最简约树。结果表明，所研究的下孢子囊菌块菌和块菌样类群代表了盘菌目中至少5个独立的谱系。结果还表明，一些上位和大多数下位分类群已被错误的分类位置。Bootstrap分析表明，这是一个单系姐妹群的羊肚菌科，Dis- cinaceae进化枝的紫穗槐科，Helvellaceae分支的强有力的支持。Rhizina在MP和ML树中是这两个分支的姐妹群，而在NJ树中它是羊肚菌科-盘菌科分支的姐妹群。MP，ML和NJ树拓扑结构表明，根应删除Helvellaceae和单型家庭，Rhizinaceae分类。本文提出了一个基于遗传学的真菌分类，并对4个科进行了订正。
Phylogenetic relationships among asco- mycetous truffles and the true and false morels were examined by using sequences from two nuclear en- coded ribosomal DNA genes. The data consist of 18S rDNA and partial 28S rDNA sequences for 29 taxa. Individual and combined data sets were analyzed by maximum parsimony (MP), neighbor-joining (NJ) and maximum likelihood (ML) methods. Parsimony analysis of the combined data set, which contained 3 published 18S sequences and consisted of 2358 nu- cleotide characters, yielded a single most parsimoni- ous tree of 1728 steps. The results indicate that the hypogeous ascomycetous truffle and trufflelike taxa studied represent at least 5 independent lineages within the Pezizales. Results also suggest that several epigeous and most hypogeous taxa have been mis- placed taxonomically. Bootstrap analyses show strong support for a Tuberaceae-Helvellaceae clade which is a monophyletic sister group of a Morchellaceae-Dis- cinaceae clade. Rhizina is a sister group to both of these clades in the MP and ML trees while in the NJ tree it is a sister of the Morchellaceae-Discinaceae clade. MP, ML, and NJ tree topologies indicate that Rhizina should be removed from the Helvellaceae and classified in a monotypic family, the Rhizinaceae. A phylogenetically-based classification for these fungi is proposed together with emendations of 4 families.