Mucosal vaccine made from live, recombinant Lactococcus lactis protects mice against pharyngeal infection with Streptococcus pyogenes
Mucosal vaccine made from live, recombinant Lactococcus lactis protects mice against pharyngeal infection with Streptococcus pyogenes
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DOI：
10.1128/iai.72.6.3444-3450.2004
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发表时间：
2004-06-01
影响因子：
3.1
通讯作者：
Geller, BL
中科院分区：
文献类型：
--
作者：
Mannam, P;Jones, KF;Geller, BL
一种由活的非致病性乳酸乳球菌制成的新型疫苗 (LL-CRR) 在小鼠中进行了测试，该疫苗表达来自化脓性链球菌血清型 6 的 M 蛋白的保守 C 重复区域 (CRR)。经鼻接种疫苗的小鼠产生 CRR 特异性唾液免疫球蛋白 A (IgA) 和血清 IgG。皮下接种疫苗的小鼠产生 CRR 特异性血清 IgG，但不产生唾液 IgA。联合方案产生的反应与鼻腔接种小鼠的唾液 IgA 和皮下接种小鼠的血清 IgG 相似。在用化脓性链球菌 M 血清型 14 进行鼻部攻击后，经鼻或联合方案接种疫苗的小鼠可显着预防咽部感染。皮下接种疫苗的小鼠不能预防咽部感染。所有三个 LL-CRR 疫苗接种组的小鼠均显着免受化脓性链球菌的致命影响。 77 只接种 LL-CRR 的攻击小鼠中只有 1 只死亡，而 118 只接种对照菌株或磷酸盐缓冲盐水的攻击小鼠中有 60 只死亡。总之，用 LL-CRR 进行粘膜疫苗接种可产生 CRR 特异性唾液 IgA 和血清 IgG，预防化脓性链球菌的咽部感染，并促进存活。
A novel vaccine (LL-CRR) made from live, nonpathogenic Lactococcus lactis that expresses the conserved C-repeat region (CRR) of M protein from Streptococcus pyogenes serotype 6 was tested in mice. Nasally vaccinated mice produced CRR-specific salivary immunoglobulin A (IgA) and serum IgG. Subcutaneously vaccinated mice produced CRR-specific serum IgG but not salivary IgA. A combined regimen produced responses similar to the salivary IgA of nasally vaccinated mice and serum IgG of subcutaneously vaccinated mice. Mice vaccinated nasally or with the combined regimen were significantly protected against pharyngeal infection following a nasal challenge with S. pyogenes M serotype 14. Mice vaccinated subcutaneously were not protected against pharyngeal infection. Mice in all three LL-CRR vaccination groups were significantly protected against the lethal effects of S. pyogenes. Only I of 77 challenged mice that were vaccinated with LL-CRR died, whereas 60 of 118 challenged mice that were vaccinated with a control strain or phosphate-buffered saline died. In conclusion, mucosal vaccination with LL-CRR produced CRR-specific salivary IgA and serum IgG, prevented pharyngeal infection with S. pyogenes, and promoted survival.