Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II.
Localization of HeLa cell tumor-suppressor gene to the long arm of chromosome II.
复制标题
DOI：
--
复制
发表时间：
1989-10
影响因子：
9.8
通讯作者：
B. C. Misra;E. Srivatsan
中科院分区：
文献类型：
--
作者：
B. C. Misra;E. Srivatsan
人类种内HeLa x成纤维细胞杂交的细胞遗传学和分子遗传学分析提供了正常细胞11号染色体上存在肿瘤抑制基因的证据。在本研究中，我们用至少50种不同的11号染色体特异性探针对各种非致瘤性和致瘤性杂交种进行了广泛的RFLP分析，以确定该肿瘤抑制基因的精确位置。两种不同的杂交系统，（1）通过将正常染色体11转移到致瘤性HeLa衍生的杂交细胞中而衍生的微细胞杂交体和（2）通过将HeLa（D98 OR）细胞融合到视网膜母细胞瘤（Y 79）细胞系中而衍生的体细胞杂交体，是特别有用的。分析表明，除了一个非致瘤性杂交细胞系外，所有杂交细胞系都含有正常11号染色体的完整拷贝。这种变异的杂交体含有一段长臂，但失去了整个染色体的短臂。致瘤微细胞和体细胞杂交体保留了染色体的短臂，但至少丢失了染色体的q13-23区域。因此，这些结果显示了11号染色体长臂的存在与肿瘤发生表型的抑制之间的完美相关性。因此，我们得出结论，参与抑制HeLa细胞肿瘤的基因定位于11号染色体的长臂（q臂）。
Cytogenetic and molecular genetic analyses of human intraspecific HeLa x fibroblast hybrids have provided evidence for the presence of a tumor-suppressor gene(s) on chromosome 11 of normal cells. In the present study, we have carried out extensive RFLP analysis of various nontumorigenic and tumorigenic hybrids with at least 50 different chromosome 11-specific probes to determine the precise location of this tumor-suppressor gene(s). Two different hybrid systems, (1) microcell hybrids derived by the transfer of a normal chromosome 11 into a tumorigenic HeLa-derived hybrid cell and (2) somatic cell hybrids derived by the fusion of the HeLa (D98OR) cells to a retinoblastoma (Y79) cell line, were particularly informative. The analysis showed that all but one of the nontumorigenic hybrid cell lines contained a complete copy of the normal chromosome 11. This variant hybrid contained a segment of the long arm but had lost the entire short arm of the chromosome. The tumorigenic microcell and somatic cell hybrids had retained the short arm of the chromosome but had lost at least the q13-23 region of the chromosome. Thus, these results showed a perfect correlation between the presence of the long arm of chromosome 11 and the suppression of the tumorigenic phenotype. We conclude therefore that the gene(s) involved in the suppression of the HeLa cell tumors is localized to the long arm (q arm) of chromosome 11.