Indirect projections from the suprachiasmatic nucleus to major arousal-promoting cell groups in rat: Implications for the circadian control of behavioural state
Indirect projections from the suprachiasmatic nucleus to major arousal-promoting cell groups in rat: Implications for the circadian control of behavioural state
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DOI：
10.1016/j.neuroscience.2004.08.030
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Semba, K
中科院分区：
文献类型：
--
作者：
Deurveilher, S;Semba, K
位于视交叉上核(SCN)的生物钟控制着各种昼夜节律，包括每天的睡眠-觉醒周期。通过双束追踪，我们最近发现，视前内侧区(MPA)、室旁区(SPVZ)和下丘脑背内侧核(DMH)可以将SCN的输出传递到两个关键的睡眠促进核--视前外侧核和视前正中核。本研究探讨了这三个核团可能将SCN与觉醒调节神经元群联系起来的可能性。将带有或不带有霍乱毒素B亚单位的生物素化葡聚糖胺注射到选定的SCN传出的主要靶点；先前对SCN的逆行标记进行了分析。在这里，顺行标记分析了免疫组织化学确定的胆碱能、增食欲素/下克汀组和胺能细胞组。将示踪剂注射到MPA、SPVZ和DMH后，食欲素区和结节乳头状核的静脉曲张纤维出现中等到密集的顺行标记。蓝斑，特别是树突区，含有中等顺行标记的MPA和DMH。腹侧被盖区、中缝背核、背侧被盖外侧核均呈中度顺行标记。无名质呈中度顺行标记。这些结果表明，MPA、SPVZ和DMH可能是SCN间接投射的中继核，不仅如先前所示那样投射到促进睡眠的视前核团，而且还投射到整个大脑的觉醒调节细胞群。在大多数睡眠/觉醒调节区缺乏主要的SCN直接投射的情况下，间接神经元通路可能在睡眠-觉醒周期和其他生理功能的昼夜控制中发挥重要作用。(C)2004年IBRO。爱思唯尔有限公司出版。保留所有权利。
The circadian clock housed in the suprachiasmatic nucleus (SCN) controls various circadian rhythms including daily sleep-wake cycles. Using dual tract-tracing, we recently showed that the medial preoptic area (MPA), subparaventricular zone (SPVZ) and dorsomedial hypothalamic nucleus (DMH) are well positioned to relay SCN output to two key sleep-promoting nuclei, namely, the ventrolateral and median preoptic nuclei. The present study examined the possibility that these three nuclei may link the SCN with wake-regulatory neuronal groups. Biotinylated dextran-amine with or without cholera toxin B subunit was injected into selected main targets of SCN efferents; the retrograde labeling in the SCN was previously analyzed. Here, anterograde labeling was analyzed in immunohistochemically identified cholinergic, orexin/hypocretin-containing and aminergic cell groups. Tracer injections into the MPA, SPVZ and DMH resulted in moderate to dense anterograde labeling of varicose fibers in the orexin field and the tuberomammillary nucleus. The locus coeruleus, particularly the dendritic field, contained moderate anterograde labeling from the MPA and DMH. The ventral tegmental area, dorsal raphe nucleus, and laterodorsal tegmental nucleus all showed moderate anterograde labeling from the DMH. The substantia innominata showed moderate anterograde labeling from the MPA. These results suggest that the MPA, SPVZ and DMH are possible relay nuclei for indirect SCN projections not only to sleep-promoting preoptic nuclei as previously shown, but also to wake-regulatory cell groups throughout the brain. In the absence of major direct SCN projections to most of these sleep/wake-regulatory regions, indirect neuronal pathways probably play an important role in the circadian control of sleep-wake cycles and other physiological functions. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.