Immunocytochemical localization of the beta I subspecies of protein kinase C in rat brain.
Immunocytochemical localization of the beta I subspecies of protein kinase C in rat brain.
复制标题
大鼠脑中蛋白激酶 C β I 亚种的免疫细胞化学定位。
DOI：
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发表时间：
1989
影响因子：
11.1
通讯作者：
Yasutomi Nishizuka
中科院分区：
文献类型：
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作者：
Kohkichi Hosoda;Naoaki Saito;A. Kose;A. Ito;T. Tsujino;K. Ogita;U. Kikkawa;Yoshitaka Ono;Koichi Igarashi;Yasutomi Nishizuka
针对含有β I蛋白激酶C（PKC）特异性氨基酸序列的寡肽，提出了多克隆抗体，以便在大鼠脑中定位PKC的这一亚种。β I PKC免疫反应广泛分布，但离散定位于特定的脑区。免疫反应性与神经元相关，但与神经胶质细胞无关，中性粒细胞中的免疫反应性通常较弱，三角隔核、脑桥核、上级丘浅层和脊髓灰质等区域除外。在三角隔核和脑桥核中，β I PKC免疫反应阳性细胞数量最多。在这些密集的β-I免疫反应性区域，没有或很少有γ-PKC免疫反应性。β-I PKC免疫反应在胞体、树突和轴突中观察到，但不在细胞核或核仁中。胞质免疫反应阳性细胞的分布形式多样，但多见于胞体周围和树突。β I PKC免疫反应性的神经元内定位不同于由γ基因序列编码的亚种。结果表明，β I PKC可能在特定神经元的细胞膜反应性的调节中发挥作用。
Polyclonal antibodies were raised against an oligopeptide containing an amino acid sequence specific for beta I protein kinase C (PKC) in order to localize this subspecies of PKC in the rat brain. beta I PKC immunoreactivity was widely distributed but discretely localized in particular brain regions. The immunoreactivity was associated with neurons but not glial cells and was generally weak in the neutrophils with the exception of areas such as the triangular septal nucleus, pontine nuclei, superficial layer of the superior colliculus, and gray matter of the spinal cord. The largest number of beta I PKC-immunoreactive cells was seen in the triangular septal nucleus and pontine nuclei. In these areas of dense beta I immunoreactivity, there was no or little gamma PKC immunoreactivity. The beta I PKC immunoreactivity was observed in perikarya, dendrites, and axons but not in nuclei or nucleoli. There were various patterns of cytoplasmic immunoreactivity, but the immunoreactivity was seen frequently in the periphery of the perikarya and dendrites. The intraneuronal localization of beta I PKC immunoreactivity differs from that of the subspecies that is coded by the gamma gene sequence. The results suggest that beta I PKC may play a role in the regulation of cell membrane responsiveness in particular neurons.