Reduction of fatty acid flux at low temperature led to enhancement ofβ-carotene biosynthesis in recombinant Saccharomycescerevisiae
Reduction of fatty acid flux at low temperature led to enhancement ofβ-carotene biosynthesis in recombinant Saccharomycescerevisiae
复制标题
低温下脂肪酸通量的减少导致重组酿酒酵母β-胡萝卜素生物合成的增强
DOI：
10.1007/s11814-014-0318-6
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Guoliang Yan
中科院分区：
文献类型：
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作者：
Liang Sun;Fei Shang;Changqing Duan;Guoliang Yan
转移重组体S.从30 °C到4 °C，β-胡萝卜素含量增加了41.4%（3.96mg/g细胞干重），并伴随着脂肪酸和麦角甾醇的积累。对甲羟戊酸途径基因转录水平的比较表明，4 °C时HMG 1、ERG 9、ERG 19、ERG 20和IDI 1的表达量均高于30 °C。这表明，增加甲羟戊酸途径基因的转录有助于低温下β-胡萝卜素产量的提高。添加30 mg/L的脂肪酸合成抑制剂三氯生后，β-胡萝卜素的含量（4.94mg/g DCW）比30 °C下增加了28.3%，比添加相同浓度三氯生时增加了18.8%。HMG、ERG 19和ERG 20的高表达水平以及麦角甾醇含量的同时增加（17.8%）表明，在低温（4 °C）下适当阻断脂肪酸合成的情况下，更多的碳源从脂肪酸合成途径转移到甲羟戊酸途径，从而导致β-胡萝卜素产量较30 °C有更高的增加。这项研究的结果共同表明，降低温度和添加脂肪酸合成抑制剂的组合是一种潜在的方法，以提高生产所需的类异戊二烯化合物，如类胡萝卜素。
Transferring the recombinantS. cerevisiaeT73-63 from 30 °C to 4 °C resulted in 41.4% increment ofβ-carotene concentration (3.96mg/g dry cell weight) relative to that of 30 °C, which was accompanied with the accumulation of fatty acid and ergosterol. The comparisons of the transcriptional levels of mevalonate pathway genes indicated that the expressions ofHMG1,ERG9,ERG19,ERG20andIDI1at 4 °C were all higher than those of 30 °C, respectively. This suggested that increased transcriptions of mevalonate pathway genes contribute to the improvement ofβ-carotene production at low temperature. We also found that supplementation of 30mg/L triclosan, an inhibitor of fatty acid synthesis, led to further 28.3% enhancement ofβ-carotene concentration (4.94mg/g DCW), which was 18.8% higher than that of 30 °C with the same concentration of triclosan. The higher expressional levels ofHMG,ERG19andERG20and the simultaneous increment of ergosterol content (17.8%) suggested that more carbon source was transferred from fatty acid synthesis to mevalonate pathway under the circumstance of appropriately blocking fatty acid synthesis at low temperature (4 °C), which resulted in a higher increment ofβ-carotene production compared to that of 30 °C. The results of this study collectively suggest that the combination of reducing temperature and adding fatty acid synthesis inhibitors is a potential approach to improve the production of desirable isoprenoid compounds such as carotenoids.