Cloning and characterisation of the cDNA for sheep liver cytosolic aldehyde dehydrogenase.
Cloning and characterisation of the cDNA for sheep liver cytosolic aldehyde dehydrogenase.
复制标题
绵羊肝细胞质醛脱氢酶 cDNA 的克隆和表征。
DOI：
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发表时间：
1995
影响因子：
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通讯作者：
J. Tweedie
中科院分区：
文献类型：
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作者：
C. Stayner;J. Tweedie
绵羊肝胞质醛脱氢酶（AIDH）已被来自该部门的其他人广泛研究（综述于Blackwell等人，1989年）。为了使用定点诱变扩展这些研究，首先有必要分离和测序这种形式的酶的cDNA。人肝细胞溶质的cDNA序列（Hsu等人，1989）和AIDH的线粒体同种型（Hsu等，1988）用于设计允许特异性鉴定胞质AIDH的探针。我们在这里报告的分离和测序的全长克隆羊肝胞质AIDH。
Sheep liver cytosolic aldehyde dehydrogenase (AIDH) has been studied extensively by others from this department (reviewed in Blackwell et al., 1989). In order to extend these studies using site-directed mutagenesis it was necessary first to isolate and sequence the cDNA for this form of the enzyme. The cDNA sequences for the human liver cytosolic (Hsu et al., 1989) and mitochondrial iso-forms of AIDH (Hsu et al., 1988) were used to design a probe which would allow specific identification of cytosolic AIDH. We report here the isolation and sequencing of a full-length clone for sheep liver cytosolic AIDH.