METABOLISM OF PYRIMIDINES AND PYRIMIDINE NUCLEOSIDES BY SALMONELLA-TYPHIMURIUM
METABOLISM OF PYRIMIDINES AND PYRIMIDINE NUCLEOSIDES BY SALMONELLA-TYPHIMURIUM
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DOI：
10.1128/jb.110.1.219-228.1972
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发表时间：
1972-01-01
影响因子：
3.2
通讯作者：
NEUHARD, J
中科院分区：
文献类型：
--
作者：
BECK, CF;THOMASSEN, E;NEUHARD, J
建立了鼠伤寒沙门氏菌代谢尿嘧啶、胞嘧啶、尿苷、胞苷、脱氧尿苷和脱氧胞苷的途径。各种5-氟嘧啶类似物仅在转化为核苷酸水平后才显示出其毒性作用，并且这些转化显示出由类似地转化天然底物的相同酶催化。描述了用于分离在嘧啶碱基和核苷代谢的各个步骤中被阻断的突变菌株的方法。
The pathways by which uracil, cytosine, uridine, cytidine, deoxyuridine, and deoxycytidine are metabolized bySalmonella typhimuriumare established. The various 5-fluoropyrimidine analogues are shown to exert their toxic effects only after having been converted to the nucleotide level, and these conversions are shown to be catalyzed by the same enzymes which similarly convert the natural substrates. Methods for isolating mutant strains blocked in various steps of metabolism of pyrimidine bases and nucleosides are described.