FLOW CYTOMETRIC RETICULOCYTE ANALYSIS - MULTIINSTITUTIONAL INTERLABORATORY CORRELATION STUDY
FLOW CYTOMETRIC RETICULOCYTE ANALYSIS - MULTIINSTITUTIONAL INTERLABORATORY CORRELATION STUDY
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DOI：
10.1093/ajcp/102.4.468
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发表时间：
1994-10-01
影响因子：
3.5
通讯作者：
BATJER, JD
中科院分区：
文献类型：
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作者：
DAVIS, BH;BIGELOW, NC;BATJER, JD
网织红细胞分析流式细胞术提供的精度和灵敏度高于传统的形态学方法，并允许推导出网织红细胞成熟指数。然而，尚未报告实验室间变异性。作者在4个月内使用11种仪器分析了8个地点的310个样本，以检查方法间和实验室间的变异性。染色剂包括噻唑橙子、溴化乙锭和金胺O。仪器包括Coulter、Becton Dickinson、TOA Medical Electronics和Ortho Diagnostics的型号。所有研究中心和方法对这些样本的变异系数（CV）随网织红细胞百分比的变化而变化，网织红细胞2.5%的样本的平均CV为69%。使用TOA R-1000专用网织红细胞分析仪观察到最佳性能，对于含2.5%网织红细胞的样本，平均CV为18.4%。网织红细胞成熟指数显示出相当的研究中心间精密度，对于网织红细胞>2.5%的样本，多功能流式细胞仪的平均CV为16%，TOA R-1000仪器的平均CV为7.3%。网织红细胞计数在所有研究中心的组间相关性范围为0.79至0.99，网织红细胞成熟指数在0.41至0.88。作者得出结论，流式细胞仪网织红细胞分析比手动网织红细胞分析更精确。随着该方法的自动化程度提高，可以实现网织红细胞计数和网织红细胞成熟指数的实验室间标准化。
Reticulocyte analysis by flow cytometry offers precision and sensitivity greater than those of conventional morphologic methods and permits derivation of a reticulocyte maturity index. However, interlaboratory variability has not yet been reported. The authors analyzed 310 samples at eight sites using 11 instruments over a 4-month period to examine intermethod and interlaboratory variabilities. Stains included thiazole orange, ethidium bromide, and auramine O. Instruments included models by Coulter, Becton Dickinson, TOA Medical Electronics, and Ortho Diagnostics. The coefficient of variation (CV) among all sites and methods on these samples varied as a function of the reticulocyte percentage, ranging from a mean CV of 69% for samples with 2.5% reticulocytes. The best performance was observed with the TOA R-1000 dedicated reticulocyte analyzers, with a mean CV of 18.4% for samples with 2.5% reticulocytes. The reticulocyte maturity index showed comparable intersite precision, with a mean CV of 16% for samples with >2.5% reticulocytes with multipurpose flow cytometers and a mean CV of 7.3% with the TOA R-1000 instruments. Interclass correlations among all sites ranged from .79 to .99 for the reticulocyte counts and .41 to .88 for the reticulocyte maturity index. The authors conclude that flow cytometric reticulocyte analysis is more precise than manual reticulocyte analysis. With greater automation of this methodology, further interlaboratory standardization of reticulocyte counts and the reticulocyte maturity index can be achieved.