Dissection of the Endolymphatic Sac from Mice.
Dissection of the Endolymphatic Sac from Mice.
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DOI：
10.3791/62375
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发表时间：
2021-03-29
期刊：
影响因子：
--
通讯作者：
Roux I
中科院分区：
文献类型：
--
作者：
Honda K;Lee HJ;Griffith AJ;Roux I
对人类听力和平衡障碍的突变小鼠模型的研究揭示了许多可能导致人类表型的结构和功能变化。虽然在理解耳蜗和前庭神经感觉上皮的发育和功能方面取得了重要进展，但关于内淋巴囊的发育、细胞组成、分子途径和功能特征的知识有限。这在很大程度上是由于难以观察和显微解剖这种组织，这种组织是仅由一个细胞层组成的上皮。本研究描述了一种从不同年龄的野生型小鼠内耳进入和显微解剖内淋巴囊的方法。类似的解剖结果显示在pendrin缺陷小鼠模型的前庭水管扩大。一个转基因小鼠与荧光内淋巴囊。该报告小鼠可用于在有限解剖的情况下容易地可视化内淋巴囊并确定其大小。它也可以作为一种教育工具，教如何解剖内淋巴囊。这些解剖过程应有助于进一步表征内耳的这一未充分研究的部分。
The study of mutant mouse models of human hearing and balance disorders has unraveled many structural and functional changes which may contribute to the human phenotypes. Although important progress has been done in the understanding of the development and function of the neurosensory epithelia of the cochlea and vestibula, limited knowledge is available regarding the development, cellular composition, molecular pathways and functional characteristics of the endolymphatic sac. This is, in large part, due to the difficulty of visualizing and microdissecting this tissue, which is an epithelium comprised of only one cell layer. The study presented here describes an approach to access and microdissect the endolymphatic sac from the wild-type mouse inner ear at different ages. The result of a similar dissection is shown in a pendrin-deficient mouse model of enlargement of the vestibular aqueduct. A transgenic mouse with a fluorescent endolymphatic sac is presented. This reporter mouse can be used to readily visualize the endolymphatic sac with limited dissection and determine its size. It can also be used as an educational tool to teach how to dissect the endolymphatic sac. These dissection procedures should facilitate further characterization of this understudied part of the inner ear.
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影响因子：
15.9
作者：
Choi, Byung Yoon;Kim, Hyoung-Mi;Griffith, Andrew J.
通讯作者：
Griffith, Andrew J.
DOI：
10.1007/s10162-002-3052-4
发表时间：
2003-09-01
影响因子：
2.4
作者：
Royaux, IE;Belyantseva, IA;Green, ED
通讯作者：
Green, ED
影响因子：
25
作者：
Madisen L;Zwingman TA;Sunkin SM;Oh SW;Zariwala HA;Gu H;Ng LL;Palmiter RD;Hawrylycz MJ;Jones AR;Lein ES;Zeng H
通讯作者：
Zeng H
影响因子：
3.7
作者：
Kim HM;Wangemann P
通讯作者：
Wangemann P
影响因子：
2.8
作者：
Griffith, Andrew J.;Wangemann, Philine
通讯作者：
Wangemann, Philine