Growth hormone short-loop feedback: anatomic specificity of growth hormone stimulation of hypothalamic somatostatin concentration.
Growth hormone short-loop feedback: anatomic specificity of growth hormone stimulation of hypothalamic somatostatin concentration.
复制标题
生长激素短环反馈：生长激素刺激下丘脑生长抑素浓度的解剖特异性。
DOI：
10.1055/s-2007-999201
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发表时间：
1980
影响因子：
2.2
通讯作者：
Hlivyak Le
中科院分区：
文献类型：
--
作者：
Molitch Me;Hlivyak Le
方法26日龄雄性Sprague-Dawley大鼠在垂体切除术后14天（Charles River Laboratories）注射12.5 μ g/天持续4天（低剂量）或50 μ g/天持续4天（高剂量）的人GH（hGH）（NIH GH-HS 1544 c），对照组注射生理盐水。最后一次给药后24小时，将大鼠断头，并在5分钟内解剖下丘脑和胰腺，并置于冰IM乙酸中。通过20号针头挤压到冰IM醋酸中，破坏下丘脑外脑。通过polytron将组织均质化，并在去除100 μ M用于蛋白质测定后煮沸5分钟，并以300 rpm旋转20分钟。将上清液冷冻、冻干，并通过放射免疫测定法测定SRIF含量（Patel和Reichlin 1978）。采用BioRad法测定蛋白质含量。
MethodsFourteen days after hypophysectomy (Charles River Laboratories) 26 day old male Sprague-Dawley rats were injected with either 12.5 Mg/day for 4 days (low dose) or 50 jug/day for 4 days (high dose) of human GH (hGH)(NIH GH-HS 1544c), controls being injected with saline. 24 hours after the last dose the rats were decapitated and within 5 minutes the hypothalamus and pancreas were dissected free and placed in iced IM acetic acid. The extra-hypothalamic brain was disrupted by extrusion through a 20 gauge needle into iced IM acetic acid. Tissues were homogenized by polytron and after removing 100 M'for protein determination were boiled for 5 minutes, and spun at 300 rpm for 20 minutes. The supernanant was frozen, lyophilized and assayed for SRIF content by radioimmunoassay (Patel and Reichlin 1978). The protein content was determined by the BioRad method.