Role of type I- and type II-interferon in expression of melanoma differentiation-associated gene-5 in HSC-3 oral squamous carcinoma cells
Role of type I- and type II-interferon in expression of melanoma differentiation-associated gene-5 in HSC-3 oral squamous carcinoma cells
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DOI：
10.2220/biomedres.35.9
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发表时间：
2014-02-01
影响因子：
1.2
通讯作者：
Kimura, Hiroto
中科院分区：
文献类型：
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作者：
Kon, Takao;Matsumiya, Tomoh;Kimura, Hiroto
黑色素瘤分化相关基因 5 (MDA-5) 和视黄酸诱导基因-I (RIG-I) 是 DExH 蛋白家族的成员，已知在诱导 I 型干扰素 (IFN) 的抗病毒反应中发挥重要作用。 MDA-5 被认为可以感知具有长 (>1 kb) 双链 RNA 的 RNA 病毒。然而，MDA-5 也由参与获得性免疫的 II 型 IFN 诱导，表明 MDA-5 的作用仍有待阐明。此外，尚未进行关于口腔区域MDA-5的研究。在这里，我们研究了 MDA-5 在源自口腔上皮细胞的 HCS-3 鳞状癌细胞中的作用。用IFN-α2b或IFN-γ处理HCS-3细胞显着诱导MDA-5以及RIG-I。 IFN-a2b在HSC-3细胞中发挥抗增殖作用，而在用IFN-γ处理的细胞中没有观察到这种作用。已知 MDA-5 与黑色素瘤中的肿瘤细胞生长有关。然而，MDA-5的过表达并没有改变HSC-3细胞的增殖，表明MDA-5与此类细胞的细胞生长无关。我们得出结论，MDA-5 是由 HSC-3 细胞中的 I 型和 II 型干扰素诱导的，这表明 MDA-5 可能在口腔免疫反应中发挥作用。
Melanoma differentiation-associated gene 5 (MDA-5) and retinoic acid-inducible gene-I (RIG-I) are members of DExH family of proteins, and known to play important roles in antiviral responses to induce type I interferons (IFNs). MDA-5 has been thought to sense RNA virus with long (>1 kb) double-stranded RNA. However, MDA-5 is also induced by type II IFN that is involved in acquired immunity, suggesting that role of MDA-5 remains to be elucidated. In addition, no study regarding MDA-5 in oral region has been performed. Here we investigated the role of MDA-5 in HCS-3 squamous carcinoma cells derived from oral epithelial cells. Treatment of HCS-3 cells with IFN-alpha 2b or IFN-gamma significantly induced MDA-5 as well as RIG-I. IFN-a2b exerted anti-proliferative effect in HSC-3 cells while no such effect was observed in the cells treated with IFN-gamma. MDA-5 is known to be associated with tumor cell growth in melanoma. However, overexpression of MDA-5 did not alter the proliferation in HSC-3 cells, indicating that MDA-5 is unrelated to the cell growth in this type of cells. We conclude that MDA-5 is induced by both type I- and type II-IFNs in HSC-3 cells, and this suggests MDA-5 may play a role in immune responses in oral cavity.