Cryomacroscopy of vitrification, Part I: A prototype and experimental observations on the cocktails VS55 and DP6.
Cryomacroscopy of vitrification, Part I: A prototype and experimental observations on the cocktails VS55 and DP6.
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DOI：
10.1089/cpt.2005.3.169
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发表时间：
2005-09-01
影响因子：
--
通讯作者：
Steif, Paul S
中科院分区：
文献类型：
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作者：
Rabin, Yoed;Taylor, Michael J;Steif, Paul S
一种新的成像设备，被称为“cryomacroscope”，在这份报告中提出。该装置旨在帮助探索与大规模玻璃化和结晶相关的热和机械效应，目前的设置旨在50 μ m至2 cm的范围内。低温显微镜不是低温显微镜的替代品，而是低温生物学家的补充工具。低温显微镜和低温显微镜的组合建议作为多尺度低温生物学研究的基础。本报告介绍了在冷冻保护剂混合物DP6和VS 55中玻璃化、结晶和断裂形成的初步结果。这些结果表明，在形成晶体的趋势，根据临界冷却和复温率测量的差示扫描量热仪（DSC）在平行的研究的基础上，一些不一致。这项研究正处于早期阶段，目前正在进行生物材料的比较研究。本报告的第二部分（配套文件）介绍了冷冻保护剂中断裂形成的结果，并讨论了促进这些断裂的机械应力。结合这些报告，可在http://www.me.cmu.edu/faculty1/rabin/CryomacroscopyImages01.htm上获得玻璃化、结晶和断裂形成的低温宏观镜检查的其他照片。
A new imaging device, termed a "cryomacroscope", is presented in this report. This device is designed to assist in exploring thermal and mechanical effects associated with large-scale vitrification and crystallization, with the current setup aimed at the range of 50 mum to 2 cm. The cryomacroscope is not intended as a substitute for the cryomicroscope, but as a complementary tool for the cryobiologist. A combination of cryomacroscopy and cryomicroscopy is suggested as a basis for multi-scale cryobiology studies. This report presents initial results on vitrification, crystallization, and fracture formation in the cryoprotectant cocktails DP6 and VS55. These results show some inconsistency in the tendency to form crystals, based on critical cooling and rewarming rates measured by means of a differential scanning calorimetric device (DSC) in parallel studies. This research is in its early stages, and comparative studies on biological materials are currently underway. Part II of this report (the companion paper) presents results for fracture formation in the cryoprotectant and discusses the mechanical stresses which promote these fractures. In conjunction with these reports, additional photos of cryomacroscopy of vitrification, crystallization, and fracture formation are available at http://www.me.cmu.edu/faculty1/rabin/CryomacroscopyImages01.htm.