Kindling induces neuropsin mRNA in the mouse brain
Kindling induces neuropsin mRNA in the mouse brain
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DOI：
10.1016/0006-8993(96)00473-8
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发表时间：
1996-07
期刊：
影响因子：
2.9
通讯作者：
A. Okabe;Y. Momota;S. Yoshida;A. Hirata;J. Ito;H. Nishino;Sadao Shiosaka
中科院分区：
文献类型：
--
作者：
A. Okabe;Y. Momota;S. Yoshida;A. Hirata;J. Ito;H. Nishino;Sadao Shiosaka
应用原位杂交组织化学方法分析了点燃癫痫发生后小鼠脑内Neuropsin mRNA的表达。在对照小鼠的边缘前皮层、体感皮层、听觉皮层、鼻周皮层、鼻内皮层和梨状皮层的第二层神经元蛋白mRNA以活动依赖的方式动态增加，而在这些区域没有神经元蛋白基因的表达。除了我们之前的研究证实了海马和杏仁核复合体mRNA的增加外，在边缘区域，如副嗅核、中隔核和外侧核、对角线带核、无形质和不动带等neuropsin mRNA也有显著的增加。基因表达的动态活性依赖性变化和neuropsin定位的位点特异性表明该分子与皮层和边缘特异性神经元重组有关。
Neuropsin mRNA expression was analyzed and mapped in the mouse brains after kindling epileptogenesis by using in situ hybridization histochemistry. Dynamic increases of the neuropsin mRNA were observed in the layer II of prelimbic, somatosensory, auditory, perirhinal, entorhinal, and piriform cortices in an activity-dependent manner, though no neuropsin gene was expressed in these areas in control mice. In addition to the confirmation of our previous studies showing increases of mRNA in the hippocampus and amygdaloid complex, there were also remarkable increases of the neuropsin mRNA in the limbic areas, such as the accessory olfactory nucleus, the medial and lateral septal nucleus, the nucleus of diagonal band, the substantia innominata and the zona incerta. The dynamic activity-dependent changes of the gene expression and the site-specificity of neuropsin localization are suggesting that this molecule is implicated in cortical- and limbic-specific neuronal reorganization.