Intracellular Staphylococcus aureus escapes the endosome and induces apoptosis in epithelial cells
Intracellular Staphylococcus aureus escapes the endosome and induces apoptosis in epithelial cells
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DOI：
10.1128/iai.66.1.336-342.1998
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发表时间：
1998-01-01
影响因子：
3.1
通讯作者：
Trumble, WR
中科院分区：
文献类型：
--
作者：
Bayles, KW;Wesson, CA;Trumble, WR
我们检测了金黄色葡萄球菌乳腺炎分离株对已建立的牛乳腺上皮细胞系（MAC-T）的侵袭，以研究细胞内存活在葡萄球菌感染持续性中的潜在作用。金黄色葡萄球菌细胞显示出MAC-T细胞的剂量依赖性侵袭和细胞内存活，感染细胞的电子显微镜检查表明，细菌通过涉及膜伪足形成的机制诱导内化，在金黄色葡萄球菌内化后2小时，MAC-T细胞表现出与基质分离、变圆、斑驳的细胞膜和细胞质空泡化，所有这些都指示细胞经历程序性细胞死亡（凋亡），BS 18小时，大多数MAC-T细胞群表现出凋亡形态。关于大小相差约180 bp增量的MAC-T细胞DNA片段的产生和感染的宿主细胞的片段化核DNA的末端脱氧核苷酸转移酶介导的dUTP切口末端标记，这些结果证明在内化S后，aurcus逃逸内体并诱导非专职吞噬细胞的凋亡。
We examined the invasion of an established bovine mammary epithelial cell line (MAC-T) by a Staphylococcus aureus mastitis isolate to study the potential role of intracellular survival in the persistence of staphylococcal infections, S, aureus cells displayed dose-dependent invasion of MAC-T cells and intracellular survival, An electron microscopic examination of infected cells indicated that the bacteria induced internalization via a mechanism involving membrane pseudopod formation and then escaped into the cytoplasm following lysis of the endosomal membrane, Two hours after the internalization of S, aureus, MAC-T cells exhibited detachment from the matrix, rounding, a mottled cell membrane, and vacuolization of the cytoplasm, all of which are indicative of cells undergoing programmed cell death (apoptosis), BS 18 h, the majority of the MAC-T cell population exhibited an apoptotic morphology, Other evidence for apoptosis,vas the generation of MAC-T cell DNA fragments differing in size by increments of approximately 180 bp and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling of the fragmented nuclear DNA of the infected host cells, These results demonstrate that after internalization S, aurcus escapes the endosome and induces apoptosis in nonprofessional phagocytes.