Phylogenetic analysis of Oryza rufipogon strains and their relations to Oryza sativa strains by insertion polymorphism of rice SINEs.
Phylogenetic analysis of Oryza rufipogon strains and their relations to Oryza sativa strains by insertion polymorphism of rice SINEs.
复制标题
DOI：
10.1266/ggs.82.217
复制
发表时间：
2007-06
影响因子：
1.1
通讯作者：
Jian-Hong Xu;Chaoyang Cheng;S. Tsuchimoto;H. Ohtsubo;E. Ohtsubo
中科院分区：
文献类型：
--
作者：
Jian-Hong Xu;Chaoyang Cheng;S. Tsuchimoto;H. Ohtsubo;E. Ohtsubo
普通野生稻（Oryza rufipogon）是栽培稻Oryza sativa的祖先，具有广泛的种内变异。本研究对O. rufipogon菌株及其与O.利用26个新鉴定的野生稻p-SINE 1基因成员，对中国栽培稻（Oryza sativa）rufipogon菌株，以及从O. sativa菌株。共分离到103株O. rufipogon和O.用一对与每个p-SINE 1成员的侧翼区域杂交的引物通过PCR检测在各个基因座上每个p-SINE 1成员的存在和缺乏。根据p-SINE 1基因的插入多态性构建的系统发育树显示，O. rufipogon和O.将sativa品系分为三类。第一组为O. rufipogon多年生种主要来自中国;紫花苜蓿属japonica株系，其包括形成独特亚组的javanica株系。第二组由几乎所有的O。rufipogon一年生品系、少数O. rufipogon多年生品系和O.紫花苜蓿属籼稻品系。这些结果支持了以前的观点，即一年生O. rufipogon起源于O. rufipogon多年生居群和O. sativa起源于O. rufipogon种群。第三类为其他多年生菌株和中间型菌株。rufipogon，其中中间型菌株与在相应基因座上没有p-SINE 1成员的假定祖先和属于具有AA基因组的其它水稻物种的那些菌株最接近。这表明O. rufipogon多年生菌株可能起源于O. rufipogon中间生态型种群。
Oryza rufipogon, the progenitor of the cultivated rice species Oryza sativa, is known by its wide intraspecific variation. In this study, we performed phylogenetic analyses of O. rufipogon strains and their relationships to O. sativa strains by using 26 newly identified p-SINE1 members from O. rufipogon strains, in addition to 23 members previously identified from O. sativa strains. A total of 103 strains of O. rufipogon and O. sativa were examined for the presence and absence of each of the p-SINE1 members at respective loci by PCR with a pair of primers that hybridize to the regions flanking each p-SINE1 member. A phylogenetic tree constructed on the basis of the insertion polymorphism of p-SINE1 members showed that O. rufipogon and O. sativa strains are classified into three groups. The first group consisted of O. rufipogon perennial strains mostly from China and O. sativa ssp. japonica strains, which included javanica strains forming a distinct subgroup. The second group consisted of almost all the O. rufipogon annual strains, a few O. rufipogon perennial strains and O. sativa ssp. indica strains. These groupings, in addition to other results, support the previous notion that annual O. rufipogon originated in the O. rufipogon perennial population, and that O. sativa originated polyphyletically in the O. rufipogon populations. The third group consisted of the other perennial strains and intermediate-type strains of O. rufipogon, in which the intermediate-type strains are most closely related to a hypothetical ancestor with no p-SINE1 members at the respective loci and to those belonging to the other rice species with the AA genome. This suggests that O. rufipogon perennial strains are likely to have originated from the O. rufipogon intermediate-ecotype population.