The effect of temperature on growth and production of paralytic shellfish poisoning toxins by the cyanobacterium Cylindrospermopsis raciborskii C10
The effect of temperature on growth and production of paralytic shellfish poisoning toxins by the cyanobacterium Cylindrospermopsis raciborskii C10
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DOI：
10.1016/j.toxicon.2004.06.005
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发表时间：
2004-10-01
期刊：
影响因子：
2.8
通讯作者：
Vásquez, M
中科院分区：
文献类型：
--
作者：
Castro, D;Vera, D;Vásquez, M
Cylindrospermopsis raciborskii 是一种蓝细菌，可产生 Cylindrospermopsisine 或麻痹性贝类中毒 (PSP) 毒素。我们研究了温度对分离自巴西淡水水库的 C. raciborskii C10 的生长和 PSP 毒素产生的影响。我们在两个不同温度下分析了 PSP 毒素的细胞外和细胞内含量：19 和 25 T。C. raciborskii C10 在这两个温度下均产生 STX、GTX2 和 GTX3。还在稳定期结束时获得的细胞内提取物中在 25 T 下检测到了 dcSTX。在 25 T 下实现的生长（通过 700 nm 光密度估计）是 19 T 下的三倍。然而，在细胞或细胞外介质中 PSP 毒素的含量没有观察到显着差异。 PSP 毒素在细胞内而不是在培养基中积累的动力学表明，PSP 毒素输出过程与细胞裂解无关。 19 T 时 PSP 毒素在细胞外的积累表明 STX 生物转化为差向异构体 GTX2 和 GTX3。 C. raciborskii C10 产生的 PSP 毒素的稳定性足够高，足以使其在 30 天（25 摄氏度）或 50 天（19 摄氏度）后在培养基中保持活性。 (C) 2004 Elsevier Ltd. 保留所有权利。
Cylindrospermopsis raciborskii is a cyanobacterium which produces either cylindrospermopsine or paralytic shellfish poisoning (PSP) toxins. We studied the effect of temperature on growth and production of PSP toxins by C. raciborskii C10, isolated from a freshwater reservoir in Brazil. We analyzed the extracellular and intracellular content of PSP toxins at two different temperatures: 19 and 25 T. C. raciborskii C10 produces STX, GTX2, and GTX3 at both temperatures.dcSTX was also detected at 25 T in the intracellular extracts obtained at the end of the stationary phase. The growth achieved at 25 T and estimated by optical density at 700 nm was three times greater than at 19 T. However, no significant differences were observed in the content of PSP toxins in either the cells or the extracellular media. The kinetics of accumulation of PSP toxins within the cells rather than in the media suggests an active PSP toxins-export process that is not related to cell lysis. The extracellular accumulation of PSP toxins at 19 T suggested a biotransformation of STX to the epimers GTX2 and GTX3. The stability of the PSP toxins produced by C. raciborskii C10 was high enough for them to remain active in the media after 30 days (at 25 degreesC) or after 50 days (at 19 degreesC). (C) 2004 Elsevier Ltd. All rights reserved.