Mouse beta defensin-1 is a functional homolog of human beta defensin-1
Mouse beta defensin-1 is a functional homolog of human beta defensin-1
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DOI：
10.1007/s003359900795
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发表时间：
1998-06-01
期刊：
影响因子：
2.5
通讯作者：
Dorin, JR
中科院分区：
文献类型：
--
作者：
Morrison, GM;Davidson, DJ;Dorin, JR
防御素是3-4 kDa的抗菌肽，已经确定了三个不同的家族：α -防御素，β -防御素和昆虫防御素。最近的研究表明，β -防御素存在于人类气道中，可能与囊性纤维化（CF）肺部疾病的发病机制有关。我们在此报告了最近发现的小鼠β -防御素基因Defb1的进一步表征，有时被称为mBD-1，它与人类气道β -防御素hBD-1同源。我们报道，Defb1在包括气道在内的多种组织中表达，并且与hBD-1相似，不受脂多糖（LPS）的上调。发现Defb1由一个16kb内含子分隔的两个小外显子组成，细胞遗传学和物理图谱将其与小鼠染色体（Chr） 8上的α防御素基因簇联系起来。功能研究表明，与hBD-1一样，Defb1对铜绿假单胞菌具有盐敏感的抗菌活性。与CF肺病相关的事实是，hBD-1和mBD-1肽对洋葱伯克氏菌都没有活性。
Defensin are 3-4 kDa antimicrobial peptides of which three distinct families have been identified: alpha-defensin, beta-defensins, and insect defensins. Recent investigations have shown that beta-defensins are present in the human airways and may be relevant to the pathogenesis of cystic fibrosis (CF) lung disease. We report here the further characterization of a recently identified mouse beta-defensin gene, Defb1, sometimes referred to as mBD-1, which is homologous to the human airway beta defensin hBD-1. We report that Defb1 is expressed in a variety of tissues including the airways and, similar to hBD-1, is not upregulated by lipopolysaccharide (LPS). Defb1 was found to consist of two small exons separated by a 16-kb intron and cytogenetic, and physical mapping linked it to the alpha defensin gene cluster on mouse Chromosome (Chr) 8. Functional studies demonstrate that, Like hBD-1, Defb1 demonstrates a salt-sensitive antimicrobial activity against Pseudomonas aeruginosa. Of relevance to CF lung disease is the fact that neither the hBD-1 nor the mBD-1 peptides are active against Burkholderia cepacia.