Sortase A Mediated Protein Modifications and Peptide Conjugations.
Sortase A Mediated Protein Modifications and Peptide Conjugations.
复制标题
DOI：
10.2174/1570163812666150903115601
复制
发表时间：
2015-11
影响因子：
--
通讯作者：
N. Voloshchuk;Danni Liang;Jun F. Liang
中科院分区：
文献类型：
--
作者：
N. Voloshchuk;Danni Liang;Jun F. Liang
生物活性肽调节许多生理过程，在某些部位作为内分泌或旁分泌信号，在其他部位作为神经递质或生长因子，并显示出对人类健康有用的特性，包括抗菌、抗真菌、抗病毒和抗肿瘤活性。虽然大多数多肽可以使用分子生物学方法生产，但它们的生产数量有限，成本高，并且在纯化和分离方面存在一些困难。此外，一些具有特殊结构的肽，如环肽，很难用生物方法生产。这对于通常需要具有许多重复功能序列的长肽的生物医学应用尤其如此。原核转肽酶Sortase A介导序列特异性肽连接，是一种新的肽修饰方法。除了肽和蛋白质修饰以提高治疗肽的稳定性和特异性外，Sortase A介导的肽连接已经扩展到更广泛的应用，如分子传感、表面修饰和生物材料。在这篇综述中，我们将重点介绍Sortase A在核酸-肽偶联物、糖基化肽、修饰蛋白/抗体和环肽等方面的药理应用。
Bioactive peptides regulate many physiological processes, acting at some sites as endocrine or paracrine signals and at others as neurotransmitters or growth factors, and show useful properties for human health, including antimicrobial, antifungal, antiviral, and antitumor activities. Although most peptides can be produced using the molecular biology approach, they are produced in limited quantities at high costs and associated with some difficulties in purification and isolation. In addition, some peptides with special structures, such as cyclic peptides, can hardly be produced by the biological method. This is especially true for the biomedical applications in which long peptides with many repeated functional sequences are usually needed. Prokaryotic transpeptidase Sortase A mediates sequence specific peptide ligation and represents a new method for peptide modifications. Besides peptide and protein modifications to improve stability and specificity of therapeutic peptides, Sortase A mediated peptide ligation has been extended to wider applications such as molecular sensing, surface modification, and biomaterials. In this review, we will focus the pharmacological applications of Sortase A for the production of nucleic acid-peptide conjugates, glycosylated peptides, modified proteins/antibodies, and cyclic peptides.