HIGH-RESOLUTION H-1-NMR SPECTROSCOPY STUDIES OF EXTRACTS OF HUMAN CEREBRAL NEOPLASMS
HIGH-RESOLUTION H-1-NMR SPECTROSCOPY STUDIES OF EXTRACTS OF HUMAN CEREBRAL NEOPLASMS
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DOI：
10.1002/mrm.1910240113
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发表时间：
1992-03-01
影响因子：
3.3
通讯作者：
SUTHERLAND, G
中科院分区：
文献类型：
--
作者：
PEELING, J;SUTHERLAND, G
高分辨率1h核磁共振光谱已被用于测量人类癫痫皮质和脑肿瘤高氯酸提取物中的代谢物（丙氨酸、N -乙酰天冬氨酸、γ -氨基丁酸、谷氨酸、谷氨酰胺、天冬氨酸、牛磺酸、甘氨酸、琥珀酸、肌酸、胆碱、肌醇和葡萄糖）的浓度。所有组织均通过手术活检获得，在热凝前切除，并立即在液氮中冷冻。肿瘤中n -乙酰天冬氨酸和γ -氨基丁酸水平的降低以及谷氨酸/谷氨酰胺比例向谷氨酰胺的转变反映了神经元的损失。肿瘤中糖代谢异常（需氧糖酵解）导致琥珀酸、谷氨酸、天冬氨酸、谷氨酰胺和肌酸水平降低，甘氨酸和丙氨酸浓度普遍升高。代谢物浓度的差异可能用于肿瘤的鉴别诊断，包括脑膜瘤的肌酸和肌醇低于星形细胞瘤。低牛磺酸可区分良性和恶性星形细胞瘤。恶性星形细胞瘤和转移性肿瘤比脑膜瘤或良性星形细胞瘤更具有区域异质性。所有获得组织的患者围手术期给予甘露醇，仅在计算机断层成像增强的肿瘤组织提取物光谱中观察到甘露醇。©1992学术出版社，Inc。
High‐resolution1H NMR spectroscopy has been used to measure the concentrations of metabolites (alanine,N‐acetylaspartate, γ‐aminobutyric acid, glutamate, glutamine, aspartate, taurine, glycine, succinate, creatine, cholines, inositol, and glucose) in perchloric acid extracts of human epileptic cortex and brain tumors. All tissue was obtained by surgical biopsy, excised before thermal coagulation, and immediately frozen in liquid nitrogen. Lower levels ofN‐acetylaspartate and γ‐aminobutyric acid and a shift in the glutamate/glutamine ratio toward glutamine in the tumors reflect neuronal loss. Abnormal glucose metabolism (aerobic glycolysis) in the tumors gives decreased levels of succinate, glutamate, aspartate, glutamine, and creatine and generally increased concentrations of glycine and alanine. Differences in metabolite concentrations that may be of use in differential tumor diagnosis include lower creatine and inositol in meningiomas than in astrocytomas. Lower taurine differentiates benign from malignant astrocytomas. Malignant astrocytomas and metastatic tumors are more regionally heterogeneous than meningiomas or benign astrocytomas. Mannitol, administered perioperatively to all patients from whom tissue was obtained, was observed only in the spectra of extracts of tissue from tumors which enhanced on computerized tomographic imaging. © 1992 Academic Press, Inc.