Vaccination with recombinant filarial paramyosin induces partial immunity to Brugia malayi infection in jirds.
Vaccination with recombinant filarial paramyosin induces partial immunity to Brugia malayi infection in jirds.
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DOI：
10.4049/jimmunol.150.5.1881
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发表时间：
1993-03
影响因子：
4.4
通讯作者：
B. Li;Ramaswamy Chandrashekar;Gary J. Weil
中科院分区：
文献类型：
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作者：
B. Li;Ramaswamy Chandrashekar;Gary J. Weil
用辐照感染性幼虫接种疫苗诱导沙鼠对马来丝虫线虫感染的部分保护性免疫。先前的研究已经表明，这种免疫刺激对重组和天然丝虫副肌球蛋白的抗体应答比正常感染后所见的强得多。为了确定重组副肌球蛋白疫苗接种是否能诱导对幼虫攻击的保护性免疫，用B免疫沙鼠。马来副肌球蛋白和麦芽糖结合蛋白（BM 5-MBP）的融合蛋白（B.马来副肌球蛋白和麦芽糖结合蛋白）、单独的MBP或重组犬恶丝虫副肌球蛋白。用100只感染性幼虫s.c.攻击动物。第二次免疫后8 wk。攻毒后16周进行尸检。接种BM 5-MBP诱导了显著的保护性免疫;与MBP对照组相比，BM 5-MBP组的成虫回收率、虫长和血液微丝蚴计数均降低。成虫回收率（43%）和雌虫长度（10%）的减少具有统计学显著性（p < 0.05）。有趣的是，用D.免疫球蛋白需要进一步的研究，以确定涉及的机制，由BM 5-MBP诱导的保护性免疫，并了解该模型中的两个密切相关的重组副肌球蛋白的差异活性。
Vaccination with irradiated infective larvae induces partial protective immunity to infection with the filarial nematode Brugia malayi in jirds. Prior studies have shown that such immunization stimulates a much stronger antibody response to recombinant and native filarial paramyosin than that seen after normal infection. To determine whether vaccination with recombinant paramyosin could induce protective immunity to larval challenge, jirds were immunized with either B. malayi paramyosin and maltose binding protein (BM5-MBP) (fusion protein of B. malayi paramyosin and maltose-binding protein), MBP alone, or recombinant Dirofilaria immitis paramyosin. Animals were challenged with 100 infective larvae s.c. 8 wk after the second immunization. Necropsies were performed 16 wk after challenge. Vaccination with BM5-MBP induced significant protective immunity; adult worm recoveries, worm lengths, and blood microfilaria counts were reduced in the BM5-MBP group relative to the MBP control group. The reductions in adult worm recoveries (43%) and female worm lengths (10%) were statistically significant (p < 0.05). Interestingly, protective immunity was not induced by immunization with D. immitis paramyosin. Additional studies are needed to identify mechanisms involved in protective immunity induced by BM5-MBP and to understand the differential activity of the two closely related recombinant paramyosin proteins in this model.