Analysis of glycosphingolipid-derived oligosaccharides by high pH anion exchange chromatography.
Analysis of glycosphingolipid-derived oligosaccharides by high pH anion exchange chromatography.
复制标题
通过高 pH 阴离子交换色谱法分析鞘糖脂衍生的寡糖。
DOI：
10.1007/bf00731225
复制
发表时间：
1994
影响因子：
3
通讯作者：
Lloyd,KO
中科院分区：
文献类型：
--
作者：
Ruan,S;Lloyd,KO
As an adjunct to existing thin layer and column chromatographic methods for the identification of glycolipids a method that utilizes the high pH anion chromatographic (HPAEC) analysis of the oligosaccharides released from the glycolipids by endoglycoceramidase has been developed. Using a Dionex Carbo Pak PA1 column and elution with a linear gradient of sodium acetate in 0.2MNaOH, the elution times of eight neutral and fourteen acidic oligosaccharides derived from glycolipids were determined. Under these conditions the neutral oligosaccharides were well separated from each other but some of the acidic oligosaccharides had overlapping elution times. The ganglioside-derived oligosaccharides could be further identified by treating them with sialidase or by mild acid hydrolysis and reanalysing the products by HPAEC. The method was applied to the analysis of mixed bovine brain gangliosides. The procedure provides an additional approach for the initial identification of glycolipids by analysing the component oligosaccharides rather than the intact glycolipids.
