PROTECTION FROM FAS-MEDIATED APOPTOSIS BY A SOLUBLE FORM OF THE FAS MOLECULE
PROTECTION FROM FAS-MEDIATED APOPTOSIS BY A SOLUBLE FORM OF THE FAS MOLECULE
复制标题
DOI：
10.1126/science.7510905
复制
发表时间：
1994-03-25
期刊：
影响因子：
56.9
通讯作者：
MOUNTZ, JD
中科院分区：
文献类型：
--
作者：
CHENG, JH;ZHOU, T;MOUNTZ, JD
Fas是一种凋亡信号受体分子，存在于多种细胞表面。分子克隆和核苷酸序列分析显示，人类Fas信使RNA变异能够编码缺乏跨膜结构域的可溶性Fas分子，因为编码该区域的外显子缺失。通过流式细胞术和免疫细胞化学分析证实可溶性Fas的表达。转染变异信使RNA的细胞上清液可阻断Fas抗体诱导的细胞凋亡。可溶性Fas水平在系统性红斑狼疮患者中升高，注射可溶性Fas的小鼠表现出自身免疫特征。
Fas is an apoptosis-signaling receptor molecule on the surface of a number of cell types. Molecular cloning and nucleotide sequence analysis revealed a human Fas messenger RNA variant capable of encoding a soluble Fas molecule lacking the transmembrane domain because of the deletion of an exon encoding this region. The expression of soluble Fas was confirmed by flow cytometry and immunocytochemical analysis. Supernatants from cells transfected with the variant messenger RNA blocked apoptosis induced by the antibody to Fas. Levels of soluble Fas were elevated in patients with systemic lupus erythematosus, and mice injected with soluble Fas displayed autoimmune features.