Selection and mechanism exploration for salt-tolerant genes in tomato
Selection and mechanism exploration for salt-tolerant genes in tomato
复制标题
番茄耐盐基因筛选及机制探索
DOI：
10.1080/14620316.2018.1486739
复制
发表时间：
2019-03-04
影响因子：
1.9
通讯作者：
Xue, Zhaohui
中科院分区：
文献类型：
--
作者：
Kou, Xiaohong;Chen, Xiuyu;Xue, Zhaohui
摘要高盐是限制植物生长和生产力的一个主要破坏性环境因素。在本研究中，我们选择了8个显着丰富的盐胁迫相关途径和17个差异表达基因的基础上RNA测序（RNA-Seq）。利用qRT-PCR技术对筛选出的关键基因进行耐盐性验证，并结合分子生物学和生理学方法，以耐盐番茄品种LA 2711和盐敏感番茄品种ZS-5为材料，探讨ASR 2-like和ASR 3-like基因的耐盐机制。结果表明，外源阿坝显著提高了盐胁迫下各生理指标，但降低了两个目的基因的转录水平，表明这两个基因以负依赖阿坝的形式在耐盐性中发挥重要作用。结合阿坝的适应模式以及目的基因与阿坝在盐胁迫响应过程中的关系，推测ASR 2和ASR 3可能通过负依赖阿坝提高ROS清除酶活性和脯氨酸含量来响应盐胁迫。这些结果为番茄的遗传改良提供了良好的资源，也为获得高抗盐番茄品种提供了理论依据。
ABSTRACT High salinity is a major destructive environmental factor limiting the growth and productivity of plants. In this study, we selected 8 significantly enriched salt stress-related pathways and 17 differentially expressed genes based on RNA sequencing (RNA-Seq). We verified the salt tolerance of the selected key genes using qRT-PCR and combined molecular biology and physiology to explore the salt tolerance mechanism of ASR2-like and ASR3-like genes using the salt-tolerant tomato cultivar LA2711 and the salt-sensitive tomato cultivar ZS-5. The results showed that exogenous ABA significantly increased all physiological indexes but decreased the transcript levels of two target genes compared with untreated controls under salt stress, suggesting that the two genes play an important role in salt tolerance in the form of negative dependence on ABA. Combined with the ABA adaptation pattern and the relationship between the target gene and ABA in the process of response to salt stress, we concluded that ASR2-like and ASR3-like might respond to salt stress by increasing the activities of ROS-scavenging enzymes and proline content via negative dependence on ABA. Taken together, these results provide a good resource for the genetic improvement of tomato and a theoretical basis for obtaining tomato varieties with high resistance to salt.