Chromogenic in situ hybridization analysis of HER-2/neu status in cytological samples of breast carcinoma
Chromogenic in situ hybridization analysis of HER-2/neu status in cytological samples of breast carcinoma
复制标题
DOI：
10.1111/j.1365-2303.2004.00214.x
复制
发表时间：
2004-12-01
期刊：
影响因子：
1.3
通讯作者：
Oh, YL
中科院分区：
文献类型：
--
作者：
Kim, GY;Oh, YL
目前，人源化单抗曲妥珠单抗用于治疗转移性乳腺癌患者，使评估HER-2/neu状态成为一个重要的临床问题。显色原位杂交是一种用免疫组织化学(IHC)样的过氧化物酶反应检测DNA探针的方法，最近发展起来用于评估福尔马林固定的乳腺癌标本中HER-2/neu的状态。我们应用HER-2/neu和17号染色体着丝粒双色探针对27例细胞学涂片进行了检测，这些细胞学标本取自新鲜乳腺肿瘤的刮片。我们还用抗HER-2/neu的单抗CB11，用免疫组化和免疫组化方法检测了HER-2/neu在相应的手术组织中的扩增和蛋白过表达。27例细胞学标本中，9例IHC阳性(2+和3+)，HER-2/neu基因扩增。13例IHC阳性病例(2+、3+)中，4例未见基因扩增。在匹配的手术样本中获得了相同的CISH技术结果。新鲜乳腺肿瘤的刮片样本提供了一种单层细胞群，特别适合于CSH。这项研究表明，细胞学涂片可能是一种很好的CSH检测替代方法。
The current use of humanized monoclonal antibody trastuzumab for the treatment of patients with metastatic breast cancer has made evaluation of HER-2/neu status an important clinical issue. Chromogenic in situ hybridization (CISH), in which the DNA probe is detected with an immunohistochemistry (IHC)-like peroxidase reaction, has been recently developed for the assessment of HER-2/neu status in formalin-fixed breast cancer specimens. We have applied the technique of dual-colour CISH using HER-2/neu and chromosome 17 centromere probes in 27 cytological smears, and these cytological samples were obtained from scrapings of fresh breast tumours. We also investigated HER-2/neu amplification and protein overexpression in the corresponding surgical tissues by CISH and IHC using the monoclonal antibody CB11. Of the 27 cytological cases, HER-2/neu gene amplification was observed in nine cases that were positive cases (2+ and 3+) for IHC. Among the 13 IHC positive cases (2+ and 3+), four of them showed no gene amplification. Identical results for the CISH technique were obtained in the matched surgical samples. The scrape samples from fresh breast tumour offer a monolayer cell population that is especially suitable for CISH. This study has shown that the cytological smear might be a good alternative for the CISH test.