Automated procedure for kinetic measurement of total triglycerides (as glycerol) in serum with the Gilford System 3500.
Automated procedure for kinetic measurement of total triglycerides (as glycerol) in serum with the Gilford System 3500.
复制标题
使用 Gilford System 3500 动态测量血清中总甘油三酯（甘油）的自动化程序。
作者：
G. Lehnus;L. Smith
在Gilford 3500计算机控制分析仪上使用了一个自动化程序，通过使用含水甘油标准间接测量血清甘油三酯。大多数酶法需要长时间的水解或笨拙的皂化。Bucolo和David的方法[clin.化学。19,476(1973)]，其中使用了脂肪酶和甘油酶。所述的动力学程序消除了血清空白的需要。它使用“Eskalab”原装试剂，通过在室温下酶解后在340 nm处测量甘油激酶反应中降低的NADH浓度，从而减少了每次测试的时间和成本。标准物质在14-S测量时间内吸光度的变化用于未知数的比率计算。使用可可靠和准确制备的稳定的含水标准，使该方法成为许多组织所需的可追溯性的理想方法。试剂空白漂移不影响结果。与人工终点法的相关系数为0.991，典型变异系数为2.25%，表明该方法准确可靠。
An automated procedure is used on the Gilford 3500 Computer-Directed Analyzer to measure serum triglycerides indirectly by using aqueous glycerol standards. Most enzymatic methods require long hydrolysis or awkward saponification. The method of Bucolo and David [clin. Chem. 19, 476 (1973)], in which lipase and glycerol kinase are used, is modified. The kinetic procedure described eliminates the need for a serum blank. It uses "Eskalab" bulk reagents and reduces both time and cost per test by measuring the decreasing NADH concentration from the glycerol kinase reaction at 340 nm after enzymatic hydrolysis at room temperature. The change in absorbance of the standard during a 14-s measuring time is used in the ratiometric calculation of the unknowns. The use of a stable aqueous standard that can be reliably and accurately prepared makes this method ideal for the traceability desired by many organizations. Reagent blank drift did not affect the results. A correlation coefficient of 0.991 for comparison with the manual endpoint method and a typical CV of 2.25% show this method to be accurate and reliable.