PCNA mRNA has a 3'UTR antisense to yellow crescent RNA and is localized in ascidian eggs and embryos.
PCNA mRNA has a 3'UTR antisense to yellow crescent RNA and is localized in ascidian eggs and embryos.
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PCNA mRNA 具有黄新月体 RNA 的 3UTR 反义，并且定位于海鞘卵和胚胎中。
DOI：
10.1006/dbio.1996.0195
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Jeffery,WR
中科院分区：
文献类型：
--
作者：
Swalla,BJ;Jeffery,WR
肌浆是一个局部的细胞质区域，在海鞘发育过程中参与决定轴、原肠形成、肌肉细胞规格和细胞分裂模式。非编码的黄新月(YC)RNA定位于肌浆中，但该转录本的功能尚不清楚。含有YC RNA 3‘区的探针与海鞘虫卵中的其他RNA杂交。用YC探针筛选柄海鞘的cDNA文库，以鉴定母体的YC相关RNA。该筛选得到了编码腹水增殖细胞核抗原(PCNA)的cDNA克隆ScYC26b。增殖细胞核抗原基因有一个长的3‘非翻译区，含有521个核苷酸序列，与YC RNA的3’端部分区域具有反义互补作用。增殖细胞核抗原基因和YC基因似乎是单拷贝的，可能在相反的DNA链上的3‘区域重叠。海鞘增殖细胞核抗原蛋白与果蝇、非洲爪哇和人类PCNAs的氨基酸同源性分别为61%、69%和71%。棒状海鞘胚胎含有母体和受精卵的PCNAs。母体的增殖细胞核抗原基因定位于外质，这是一个分离到胚胎发育过程中广泛增殖的细胞系的细胞质区域，而在肌浆中被耗尽，肌浆被分离到分裂较少的细胞系。合子增殖细胞核抗原mRNA仅限于发育中的神经系统，在神经细胞停止增殖后仍大量存在。用PC10单抗检测到的增殖细胞核抗原蛋白也被排除在肌浆中。这些结果表明，3‘端非编码区对YC RNA具有反义和互补作用，提示胚胎分化细胞的增殖可能受到母体增殖细胞核抗原基因和蛋白定位的限制。此外，受精性增殖细胞核抗原可能在蝌蚪幼虫的神经发育中具有新的作用。
The myoplasm is a localized cytoplasmic region that is involved in axis determination, gastrulation, muscle cell specification, and the pattern of cell divisions during ascidian development. The noncoding yellow crescent (YC) RNA is localized in the myoplasm, but the function of this transcript is unknown. Probes containing the 3′ region of YC RNA hybridize to other RNAs in ascidian eggs. A cDNA library from the ascidianStyela clavawas screened with a YC probe to identify maternal YC-related RNAs. This screen resulted in isolation of ScYC26b, a cDNA clone encoding the ascidian proliferating cell nuclear antigen (PCNA). The PCNA mRNA has a long 3′ untranslated region containing a 521-nucleotide sequence with antisense complementarity to part of the 3′ region of YC RNA. The PCNA and YC genes appear to be single copy and may overlap in their 3′ regions on opposite DNA strands. The ascidian PCNA protein has 61, 69, and 71% amino acid identity to theDrosophila, Xenopus,and human PCNAs, respectively.S. clavaembryos contain maternal and zygotic PCNA mRNAs. Maternal PCNA mRNA is localized in the ectoplasm, a cytoplasmic region that is segregated to cell lineages that proliferate extensively during embryogenesis, and is depleted in the myoplasm, which is segregated to cell lineages that undergo fewer divisions. Zygotic PCNA mRNA is confined to the developing nervous system and is still abundant after the neural cells have ceased to proliferate. PCNA protein, detected with PC10 monoclonal antibody, is also excluded from the myoplasm. These results show that the 3′ UTR of PCNA mRNA is antisense and complementary to YC RNA and suggest that differential cell proliferation in the embryo may be limited by localization of maternal PCNA mRNA and protein. Furthermore, zygotic PCNA may have a novel role in neural development in the tadpole larva.