Distribution Analysis of Anthocyanins, Sugars, and Organic Acids in Strawberry Fruits Using Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry
Distribution Analysis of Anthocyanins, Sugars, and Organic Acids in Strawberry Fruits Using Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry
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DOI：
10.1021/acs.jafc.8b00853
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发表时间：
2018-05-16
影响因子：
6.1
通讯作者：
Yamane, Hisakazu
中科院分区：
文献类型：
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作者：
Enomoto, Hirofumi;Sato, Kei;Yamane, Hisakazu
花青素，糖和有机酸有助于草莓的外观，健康益处和味道。然而，它们在成熟果实中的空间分布尚未完全揭示。因此，我们进行了基质辅助激光解吸/电离，MALDI-IMS，分析，以调查其在成熟草莓的空间分布。通过使用TM-喷雾器进行基质施用，提高了检测灵敏度。在花托中，天竺葵素分布在皮肤、皮层和髓组织中，而矢车菊素和飞燕草素轻微地定位在皮肤中。瘦果中花青素主要分布在果皮的外侧。柠檬酸主要分布在皮层组织的上、下两侧。虽然己糖几乎均匀地分布在整个果实中，但蔗糖主要分布在皮层和髓组织的上部。这些结果表明，使用TM-喷雾器在MALDI-IMS是有用的微观分布分析的花色素苷，糖，和有机酸的草莓。
Anthocyanins, sugars, and organic acids contribute to the appearance, health benefits, and taste of strawberries. However, their spatial distribution in the ripe fruit has been fully unrevealed. Therefore, we performed matrix-assisted laser desorption/ionization, MALDI-IMS, analysis to investigate their spatial distribution in ripe strawberries. The detection sensitivity was improved by using the TM-Sprayer for matrix application. In the receptacle, pelargonidins were distributed in the skin, cortical, and pith tissues, whereas cyanidins and delphinidins were slightly localized in the skin. In the achene, mainly cyanidins were localized in the outside of the skin. Citric acid was mainly distributed in the upper and bottom side of cortical tissue. Although hexose was distributed almost equally throughout the fruits, sucrose was mainly distributed in the upper side of cortical and pith tissues. These results suggest that using the TM-Sprayer in MALDI-IMS was useful for microscopic distribution analysis of anthocyanins, sugars, and organic acids in strawberries.