Two-stage chemical oncogenesis in cultures of C3H/10T1/2 cells.
Two-stage chemical oncogenesis in cultures of C3H/10T1/2 cells.
复制标题
C3H/10T1/2 细胞培养物中的两阶段化学肿瘤发生。
作者：
S. Mondal;D. Brankow;C. Heidelberger
在培养的C3 H/10 T1/2小鼠胚胎细胞中已经证实了化学致癌的起始和促进阶段。用亚有效浓度的3-甲基胆蒽、苯并（a）芘或7，12-二甲基苯并（a）蒽处理细胞，4天后用非转化量的十四烷酰基佛波醇乙酸酯（TPA）、佛波醇二癸酸酯或4-α-佛波醇二癸酸酯处理细胞，产生转化。佛波醇无效。TPA不选择转化细胞。当TPA处理之前3-甲基胆蒽，没有观察到增强的转化。当TPA在烃处理后立即加入时，存在转化的显著抑制。TPA没有发挥促进活性时，烃在足够高的浓度，以产生可观的转化。TPA的促进作用不能仅仅归因于刺激细胞分裂。
The initiation and promotion stages of chemical oncogenesis have been demonstrated in cultured C3H/10T1/2 mouse embryo cells. Treatment of the cells with a subeffective concentration of 3-methylcholanthrene, benzo(a)pyrene, or 7,12-dimethylbenz(a)anthracene, followed 4 days later by a nontransforming amount of tetradecanoylphorbol acetate (TPA), phorbol didecanoate, or 4-alpha-phorbol didecanoate, produced transformation. Phorbol was ineffective. TPA did not select for transformed cells. When TPA treatment preceded 3-methylcholanthrene, no enhancement of transformation was observed. When TPA was added immediately after hydrocarbon treatment, there was a significant inhibition of transformation. TPA did not exert promoting activity when the hydrocarbons were in high enough concentrations to produce appreciable transformation. The promoting action of TPA cannot be attributed only to a stimulation of cell division.