Metabolomic estimation of the diagnosis of hepatocellular carcinoma based on ultrahigh performance liquid chromatography coupled with time-of-flight mass spectrometry.
Metabolomic estimation of the diagnosis of hepatocellular carcinoma based on ultrahigh performance liquid chromatography coupled with time-of-flight mass spectrometry.
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DOI：
10.1039/c7ra13616a
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发表时间：
2018-02-28
期刊：
影响因子：
3.9
通讯作者：
Zhang, Ai-Hua
中科院分区：
文献类型：
--
作者：
Li, Yuan-Feng;Qiu, Shi;Gao, Li-Juan;Zhang, Ai-Hua
代谢组学已被证明是生物标志物筛选、途径表征和疾病诊断的有效工具。肝细胞癌的代谢特征可能使发现新的生物标志物用于其诊断。在这项工作中，代谢组学被用来研究肝癌患者的代谢变化。采用高分辨超高效液相色谱-质谱法对肝癌患者和年龄匹配的健康对照的血浆样品进行分析，并用模式识别方法分析代谢差异。共鉴定出23种可区分的代谢物。代谢途径的改变与精氨酸和脯氨酸代谢、甘氨酸、丝氨酸和苏氨酸代谢、类固醇激素生物合成、淀粉和蔗糖代谢等有关。为了证明血浆生物标志物在肝癌诊断中的作用，选择了5种代谢产物：脱氧胆酸、3-葡萄糖醛酸苷、6-羟基肌醇葡萄糖醛酸苷、4-甲氧基肉桂酸、11b-羟孕酮和4-羟基维甲酸作为候选生物标志物。这些有助于联合模型的代谢物可以显著提高肝细胞癌的诊断性能。它已被证明是发现新的疾病检测生物标记物的强大工具，并表明代谢物小组可能对将我们的发现转化为临床有用的诊断测试具有价值。代谢组学已被证明是生物标志物筛选、途径表征和疾病诊断的有效工具。
Metabolomics has been shown to be an effective tool for biomarker screening and pathway characterization and disease diagnosis. Metabolic characteristics of hepatocellular carcinoma (HCC) may enable the discovery of novel biomarkers for its diagnosis. In this work, metabolomics was used to investigate metabolic alterations of HCC patients. Plasma samples from HCC patients and age-matched healthy controls were investigated using high resolution ultrahigh performance liquid chromatography-mass spectrometry and metabolic differences were analyzed using pattern recognition methods. 23 distinguishable metabolites were identified. The altered metabolic pathways were associated with arginine and proline metabolism, glycine, serine and threonine metabolism, steroid hormone biosynthesis, starch and sucrose metabolism, etc. To demonstrate the utility of plasma biomarkers for the diagnosis of HCC, five metabolites comprising deoxycholic acid 3-glucuronide, 6-hydroxymelatonin glucuronide, 4-methoxycinnamic acid, 11b-hydroxyprogesterone and 4-hydroxyretinoic acid were selected as candidate biomarkers. These metabolites that contributed to the combined model could significantly increase the diagnostic performance of HCC. It has proved to be a powerful tool in the discovery of new biomarkers for disease detection and suggest that panels of metabolites may be valuable to translate our findings to clinically useful diagnostic tests. Metabolomics has been shown to be an effective tool for biomarker screening and pathway characterization and disease diagnosis.
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影响因子：
4.6
作者：
Liang Q;Liu H;Wang C;Li B
通讯作者：
Li B
影响因子：
3.9
作者：
Liang, Qun;Liu, Han;Ai, Huazhang
通讯作者：
Ai, Huazhang
影响因子：
3.7
作者：
Frades I;Andreasson E;Mato JM;Alexandersson E;Matthiesen R;Martínez-Chantar ML
通讯作者：
Martínez-Chantar ML
影响因子：
--
作者：
Jové M;Collado R;Quiles JL;Ramírez-Tortosa MC;Sol J;Ruiz-Sanjuan M;Fernandez M;de la Torre Cabrera C;Ramírez-Tortosa C;Granados-Principal S;Sánchez-Rovira P;Pamplona R
通讯作者：
Pamplona R
影响因子：
3.9
作者：
Liang, Qun;Liu, Han;Zhang, Ai-Hua
通讯作者：
Zhang, Ai-Hua