Effect of busulfan on antibody production and skin allograft survival in the rat.
Effect of busulfan on antibody production and skin allograft survival in the rat.
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白消安对大鼠抗体产生和同种异体皮肤移植物存活的影响。
DOI：
10.1093/jnci/53.6.1775
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
P. Tutschka
中科院分区：
文献类型：
--
作者：
G. Santos;P. Tutschka
在不同时间给Sprague-Dawley和Lewis （LEW）大鼠注射羊红细胞与单次注射悬浮在羧甲基纤维素中的丁硫丹（BU）[≈平均致死剂量（LD50）的60%]。与布鲁里溃疡治疗相关的免疫接种时间无关，布鲁里溃疡治疗提高了SO大鼠的生理盐水凝集素滴度。即使给予超致死剂量的布鲁里溃疡，LEW大鼠也有这种辅助作用。虽然不像单独使用布鲁里菌产生的佐剂作用那么大，但使用羧甲基纤维素和经过布鲁里菌预处理的供体脾脏匀浆均可产生佐剂作用。不同时间给予BN、ACI或F344大鼠同种异体皮肤移植的LEW和（LEW × BN） f1大鼠与单剂量BU（≈LD50的60%）相比，没有显示出同种异体移植存活时间的延长或缩短。然而，当在注射BU后4小时将BN大鼠的皮肤移植物置于LEW动物身上时，同种异体移植物的存活时间最小，但明显缩短。
Sprague-Dawley and Lewis (LEW) rats were given injections of sheep red blood cells at various times in relation to a single injection of busulfan (BU) [≈ 60% of mean lethal dose (LD50)] suspended in carboxymethyl cellulose. BU treatment raised saline agglutinin titers in SO rats regardless of the time of immunization in relation to BU treatment. LEW rats had this adjuvant effect even when supralethal doses of BU were given. Although not as great as adjuvant effects produced by BU alone, adjuvant effects were produced with carboxymethyl cellulose as well as with homogenates of spleens from BU-pretreated donors. LEW and (LEW × BN)F1rats given skin allografts from BN, ACI, or F344 rats at various times in relation to a single dose of BU (≈ 60% of the LD50) did not show a lengthening or shortening of allograft survival. However, when skin grafts from BN rats were placed on LEW animals 4 hours after receiving the injection of BU, allograft survival was minimally, but significantly, shortened.