Lipoprotein cholesterol concentrations in the plasma of human subjects as measured in the fed and fasted states.
Lipoprotein cholesterol concentrations in the plasma of human subjects as measured in the fed and fasted states.
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在进食和禁食状态下测量人类受试者血浆中的脂蛋白胆固醇浓度。
DOI：
10.1093/clinchem/34.12.2456
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发表时间：
1988
影响因子：
9.3
通讯作者：
E. Schaefer
中科院分区：
文献类型：
--
作者：
J. Cohn;J. Mcnamara;E. Schaefer
血浆中的脂蛋白胆固醇浓度通常使用Friedewald公式来估计，根据该公式，极低密度脂蛋白胆固醇(VLDL-C)估计为血浆甘油三酯浓度的五分之一。通常情况下，这个公式只适用于禁食状态下患者的血浆。为了确定非禁食受试者的血浆脂蛋白胆固醇测量是否发生了实质性变化，我们采集了22名健康受试者(9名男性，13名女性，年龄22-79岁)的餐后血样，他们吃了高脂肪的食物(每公斤体重1克脂肪)。所有受试者的血浆甘油三酯浓度均在餐后升高(3h为基线的233+/-16%)。血浆中的平均胆固醇浓度基本没有变化。高密度脂蛋白胆固醇显著降低(3h为94+/-2%，P<0.001)。用Friedewald公式估算极低密度脂蛋白胆固醇(VLDL-C)和低密度脂蛋白胆固醇(LDL-C)，并与改良脂质研究诊所(LRC)方法获得的测量结果进行比较。无论哪种方法，在高脂饮食后，极低密度脂蛋白-C显著升高，低密度脂蛋白-C显著降低。用Friedewald公式估计的这些餐后变化明显大于LRC方法(P<0.01)。我们的结论是：(A)进食受试者测得的脂蛋白胆固醇浓度与禁食受试者测得的脂蛋白胆固醇浓度显著不同，(B)如果要准确评估个体患冠心病的风险，必须在禁食至少12小时后获得血浆。
Lipoprotein cholesterol concentrations in plasma are routinely estimated by using the Friedewald formula, whereby very-low-density lipoprotein cholesterol (VLDL-C) is estimated to be one-fifth the plasma triglyceride concentration. Ordinarily, this formula is applied only to plasma sampled from patients in the fasted state. To determine whether lipoprotein cholesterol measurements are altered substantially in plasma sampled from nonfasting subjects, we obtained postprandial blood samples from 22 healthy subjects (nine men, 13 women, ages 22-79 years) fed a fat-rich meal (1 g fat per kilogram body wt.). The plasma triglyceride concentration increased postprandially in all subjects (233 +/- 16% of baseline at 3 h). The mean cholesterol concentration in plasma was essentially unchanged. High-density lipoprotein cholesterol (HDL-C) was significantly decreased (94 +/- 2% at 3 h, P less than 0.001). VLDL-C and low-density lipoprotein cholesterol (LDL-C), estimated by the Friedewald formula, were compared with measurements obtained by modified Lipid Research Clinics (LRC) methodology. As measured by either method, VLDL-C increased and LDL-C decreased significantly after the fat-rich meal. These postprandial changes were significantly greater (P less than 0.01) when estimated by the Friedewald formula than by LRC methodology. We conclude that (a) lipoprotein cholesterol concentrations measured in the fed subject differ significantly from those measured in the fasted subject, and (b) plasma must be obtained after at least a 12-h fast if an individual's risk of coronary heart disease is to be accurately assessed.
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DOI：
--
发表时间：
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期刊：
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发表时间：
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作者：
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