Immuno-modulatory effects of vitamin D3 in human monocyte and macrophages
Immuno-modulatory effects of vitamin D3 in human monocyte and macrophages
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DOI：
10.1016/j.cellimm.2012.10.009
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发表时间：
2012-11-01
影响因子：
4.3
通讯作者：
Malaguarnera, L.
中科院分区：
文献类型：
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作者：
Di Rosa, M.;Malaguarnera, G.;Malaguarnera, L.
维生素D3[1α，25-(OH)(2)D-3]参与体内钙稳态的调节，促进未成熟髓系前体细胞向单核/巨噬细胞分化。在这项研究中，我们比较了1α，25-(OH)(2)D-3与肿瘤坏死因子(TNF)-α或脂多糖(LPS)在新鲜分离的人单核细胞(MonoT0)和培养7天的巨噬细胞(MOT7)中对白细胞介素1(IL)-1β、(IL)-6、TNF-α、Toll样受体(TLR)-2和(TLR)-4表达的调节作用。此外，我们还检测了1α，25-(OH)(2)D-3对巨噬细胞趋化作用的影响。用实时荧光定量RT-PCR检测MonoT0和MOT7中IL-1β、IL-6和TNF-α以及TLR-2和TLR-4的表达。用水平趋化性琼脂糖点阵法分析巨噬细胞的趋化作用，发现1α，25(OH)(2)D-3影响巨噬细胞的趋化作用，在单核/巨噬细胞成熟的两个不同阶段对IL-1β、IL-6、TNF-α和TLRs的表达有不同的调节作用。总之，我们的数据增加了关于1α，25-(OH)(2)D-3在人类单核/巨噬细胞中炎症介质表达的作用的新信息，这是这些细胞复杂功能的基础。研究MonoT0和MOT7产生的免疫介质表达模式的差异，可能为研究它们的生化和分子功能提供新的途径，并可能构成一个关于先天性免疫反应中早期或迟发事件的明确行为的模型系统。(C)2012 Elsevier Inc.保留所有权利。
Vitamin D3 [1 alpha,25-(OH)(2)D-3], involved in the regulation of body calcium homeostasis, promotes immature myeloid precursor cells differentiation into monocytes/macrophages. In this study we compared the regulatory interaction between 1 alpha,25-(OH)(2)D-3 and tumor necrosis factor (TNF)-alpha, or lipopolysaccharide (LPS) in the mRNA expression of interleukin (IL)-1 beta, (IL)-6, TNF-alpha, toll like receptors (TLR)-2 and (TLR)-4 in freshly isolated human monocyte (MonoT0) and in macrophages cultured for seven days (MOT7). Additionally, we detected the effect of 1 alpha,25-(OH)(2)D-3 on macrophages chemotaxis. The expression of IL-1 beta, IL-6 and TNF-alpha, as well as TLR-2 and TLR-4 in MonoT0 and in MOT7 was examined by real time RT-PCR. Macrophages chemotaxis was analyzed by using horizontal chemotaxis agarose spot assay.We found that 1 alpha,25-(OH)(2)D-3 influences macrophages chemotaxis and differently modulates the expression of IL-1 beta, IL-6, TNF-alpha and TLRs in the two different stages of monocytes/macrophage maturation. In conclusion our data add new information about the role of 1 alpha,25-(OH)(2)D-3 on the expression of inflammatory mediators in human monocyte/macrophages, underlying the complex function of these cells. Investigating the differences in the pattern of expression of immune-mediators produced by MonoT0 and MOT7 may provide a new way to examine their biochemical and molecular function and may constitute a model system with well-defined behavior with respect to early or tardive events in the innate immune response. (C) 2012 Elsevier Inc. All rights reserved.