Epididymosomes Convey Different Repertoires of MicroRNAs Throughout the Bovine Epididymis
Epididymosomes Convey Different Repertoires of MicroRNAs Throughout the Bovine Epididymis
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DOI：
10.1095/biolreprod.113.110486
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发表时间：
2013-08-01
影响因子：
3.6
通讯作者：
Sullivan, Robert
中科院分区：
文献类型：
--
作者：
Belleannee, Clemence;Calvo, Ezequiel;Sullivan, Robert
附睾体是由附睾上皮细胞分泌的小膜泡，参与睾丸后精子的成熟。虽然它们在蛋白质转移到精子膜中的作用是有据可查的，但我们报告了它们转运microRNA（miRNAs）的能力，miRNAs是转录后基因表达的有效调节因子。使用微灌注技术结合全球微阵列的方法，我们证明，从两个离散的牛附睾区域（头和尾）的附睾体具有不同的miRNA签名。此外，我们还建立了包含在附睾体中的miRNA库与其亲本上皮细胞的miRNA库不同，这表明从细胞释放的miRNA群体可以被选择性地分选。通过流式细胞术测量DilC12标记的附睾体与原代培养的附睾细胞的结合，结果表明，来自中间头的附睾体及其miRNA含量可能掺入远端头上皮细胞。总之，这些发现揭示了不同的miRNA库以区域特异性方式释放到管腔内液体中，并且可能参与通过附睾体在整个附睾中进行细胞间通讯的新机制。
Epididymosomes are small membrane vesicles that are secreted by epididymal epithelial cells and are involved in posttesticular sperm maturation. Although their role in protein transfer to the sperm membrane is well documented, we report their capacity to transport microRNAs (miRNAs), which are potent regulators of posttranscriptional gene expression. Using a microperfusion technique combined with a global microarray approach, we demonstrated that epididymosomes from two discrete bovine epididymal regions (caput and cauda) possess distinct miRNA signatures. In addition, we also established that miRNA repertoires contained within epididymosomes differ from those of their parent epithelial cells, suggesting that miRNA populations released from the cells may be selectively sorted. Binding of DilC12-labeled epididymosomes to primary cultured epididymal cells was measured by flow cytometry, and the results indicated that epididymosomes from the median caput and their miRNA content may be incorporated into distal caput epithelial cells. Overall, these findings reveal that distinct miRNA repertoires are released into the intraluminal fluid in a region-specific manner and could be involved in a novel mechanism of intercellular communication throughout the epididymis via epididymosomes.