Relation of pH to preservative effectiveness I. Acid media
Relation of pH to preservative effectiveness I. Acid media
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pH 值与防腐效果的关系 I. 酸性介质
DOI：
10.1002/jps.2600500907
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发表时间：
1961
影响因子：
3.8
通讯作者：
D. N. Entrekin
中科院分区：
文献类型：
--
作者：
D. N. Entrekin
本研究使用的培养基为triticase豆汤，pH分别调整为2、3、4、5和6，并加入McIlvane缓冲液。从含有革兰氏阳性和革兰氏阴性杆状菌、球菌、酵母和真菌的土壤样品中获得接种物的微生物。一些防腐材料以前已经在有限的规模上进行了研究，对羟基苯甲酸盐被用作比较的基础。在理论有效范围内使用了三种浓度的防腐剂。记录了在每种浓度和pH值下的可见生长、抑菌或杀菌效果，并与对照管进行比较。一些较知名的防腐剂的有效性得到了证实，而一些较新的材料属于有效类别，而其他材料似乎无效。
Tripticase soy broth adjusted to pH 2, 3, 4, 5, and 6 with McIlvane buffer was used as media for this study. Microorganisms for the inoculum were obtained from a soil sample which contained Gram-positive and Gram-negative rods, cocci, yeast, and fungi. Some of the preservative materials have been previously investigated on a limited scale and the p-hydroxybenzoates were used as a basis for comparison. The preservative materials were used in three concentrations spanning their theoretical effective range. A record was made of visible growth, bacteriostatic or bactericidal effect at each concentration and pH value and these were compared to control tubes. Effectiveness of some of the better known preservatives was confirmed while some of the newer materials fell into the effective class and others seeming to be ineffective.