Influence of cyclic strain and decorin deficiency on 3D cellularized collagen matrices
Influence of cyclic strain and decorin deficiency on 3D cellularized collagen matrices
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DOI：
10.1016/j.biomaterials.2008.03.018
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发表时间：
2008-06-01
期刊：
影响因子：
14
通讯作者：
Grande-Allen, Kathryn J.
中科院分区：
文献类型：
--
作者：
Ferdous, Zannatul;Lazaro, Luis D.;Grande-Allen, Kathryn J.
环状菌株引起富含亮氨酸的小蛋白聚糖核心蛋白聚糖和双糖链蛋白聚糖在2D培养物和天然组织中的表达。然而，这些蛋白聚糖的应变依赖性表达尚未在工程组织中得到证实。我们推测，核心蛋白聚糖的缺乏可能会损害循环应变对工程组织发育的影响。因此，我们研究了相对于静态培养的对照组，核心蛋白聚糖对循环应变胶原凝胶中组织组织结构的贡献。将核心蛋白聚糖空（Dcn（-/-））和野生型鼠胚胎成纤维细胞接种在胶原凝胶内，并使用Flexcell（R）组织培养系统机械调节。8天后，两种细胞类型的循环应变样本显示出更大的胶原纤维密度、蛋白聚糖含量和材料强度。另一方面，仅在接种Dcn（-/-）细胞的循环应变凝胶中观察到细胞密度、胶原纤维直径和双糖链蛋白聚糖表达的增加。尽管无论细胞类型如何，循环应变引起蛋白聚糖表达的升高，但蛋白聚糖的类型在组间不同：Dcn（-/-）细胞接种的凝胶产生了野生型对照中未发现的过量的biglycan。这些结果表明，核心蛋白聚糖介导的组织的组织结构是强烈依赖于组织类型和机械环境。(c)2008爱思唯尔有限公司保留所有权利。
Cyclic strain evokes the expression of the small leucine-rich proteoglycans decorin and biglycan in 2D cultures and native tissues. However, strain-dependent expression of these proteoglycans has not been demonstrated in engineered tissues. We hypothesized that the absence of decorin may compromise the effect of cyclic strain on the development of engineered tissues. Thus, we investigated the contribution of decorin to tissue organization in cyclically strained collagen gels relative to statically cultured controls. Decorin null (Dcn(-/-)) and wild-type murine embryonic fibroblasts were seeded within collagen gels and mechanically conditioned using a Flexcell (R) Tissue Train (R) culture system. After 8 days, the cyclically strained samples demonstrated greater collagen fibril density, proteoglycan content, and material strength for both cell types. On the other hand, increases in cell density, collagen fibril diameter, and biglycan expression were observed only in the cyclically strained gels seeded with Dcn(-/-) cells. Although cyclic strain caused an elevation in proteoglycan expression regardless of cell type, the type of proteoglycan differed between groups: the Dcn(-/-) cell-seeded gels produced an excess of biglycan not found in the wild-type controls. These results suggest that decorin-mediated tissue organization is strongly dependent upon tissue type and mechanical environment. (c) 2008 Elsevier Ltd. All rights reserved.