Collagen orientation in periosteum and perichondrium is aligned with preferential directions of tissue growth
Collagen orientation in periosteum and perichondrium is aligned with preferential directions of tissue growth
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DOI：
10.1002/jor.20586
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发表时间：
2008-09-01
影响因子：
2.8
通讯作者：
Ito, Keita
中科院分区：
文献类型：
--
作者：
Foolen, Jasper;van Donkelaar, Corrinus C.;Ito, Keita
生长中的骨骼中不同组织之间的反馈机制被认为决定了骨骼的形态发生。软骨生长使周围组织紧张，引起其基质的变化，进而产生各向异性应力，引导软骨生长的方向性。这项研究的目的是通过确定软骨膜和骨膜中的胶原纤维方向是否与长骨生长的优先方向一致来评估这一假说。利用光学投影断层扫描技术(OPT)对不同发育阶段的鸡胚进行了胫骨的扫描，以评估软骨膜和骨膜特征部位的优先生长方向。将量化的形态测量数据与双光子激光扫描显微镜图像进行比较，显示这些纤维组织中的三维胶原网络。骨干骨膜含有纵向取向的胶原纤维，沿优先生长方向排列。两干骺端的纵向生长是周向生长的两倍。这与发育良好的环状纤维是一致的，它们覆盖并部分交织在干骺端软骨膜/骨膜外层的优势纵向定向纤维网络中。对于这两个关节，骨痂表面的胶原网络是随机定向的，生长大致是双轴的。这些发现支持一种假设，即骨膜和软骨膜中检测到的胶原网的各向异性结构与评估的生长方向一致。(C)2008年骨科研究会。由威利期刊出版公司出版。
A feedback mechanism between different tissues in a growing bone is thought to determine the bone's to morphogenesis. Cartilage growth strains the surrounding tissues, eliciting alterations of its matrix, which in turn, creates anisotropic stresses, guiding directionality of cartilage growth. The purpose of this study was to evaluate this hypothesis by determining whether collagen fiber directions in the perichondrium and periosteum align with the preferential directions of long bone growth. Tibiotarsi from chicken embryos across developmental stages were scanned using optical projection tomography (OPT) to assess preferential directions of growth at characteristic sites in perichondrium and periosteum. Quantified morphometric data were compared with two-photon laser-scanning microscopy images of the three-dimensional collagen network in these fibrous tissues. The diaphyseal periosteum contained longitudinally oriented collagen fibers that aligned with the preferential growth direction. Longitudinal growth at both metaphyses was twice the circumferential growth. This concurred with well-developed circumferential fibers, which covered and were partly interwoven with a dominant network of longitudinally oriented fibers in the outer layer of the perichondrium/periosteum at the metaphysis. Toward both articulations, the collagen network of the epiphyseal surface was randomly oriented, and growth was approximately biaxial. These findings support the hypothesis that the anisotropic architecture of the collagen network, detected in periosteum and perichondrium, concurs with the assessed growth directions. (C) 2008 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.