STUDIES ON ICE-NUCLEATING MICROORGANISMS .1. IDENTIFICATION OF AN ICE-NUCLEATING BACTERIUM KUIN-1 AS PSEUDOMONAS-FLUORESCENS AND ITS ICE NUCLEATION PROPERTIES
STUDIES ON ICE-NUCLEATING MICROORGANISMS .1. IDENTIFICATION OF AN ICE-NUCLEATING BACTERIUM KUIN-1 AS PSEUDOMONAS-FLUORESCENS AND ITS ICE NUCLEATION PROPERTIES
复制标题
DOI：
10.1080/00021369.1987.10868291
复制
发表时间：
1987-07-01
期刊：
影响因子：
--
通讯作者：
HIGASHI, Y
中科院分区：
文献类型：
--
作者：
OBATA, H;SAEKI, Y;HIGASHI, Y
从菜豆（Phaseolus vulgaris L.）叶子中分离得到一株冰核菌株KUIN-1。根据其分类学特征，将菌株KUIN-1鉴定为荧光假单胞菌。当菌株KUIN-1在含有Koser柠檬酸肉汤(pH 7.0)的培养基中于18℃需氧培养24小时时获得冰成核活性。 C.直到菌体浓度达到107～108/ml时才出现冰核活性。成核温度 - 3.0°在含有Koser柠檬酸盐肉汤的培养基上生长的细胞悬浮液（1.8×109细胞/ml）中检测到C。菌株 KUIN-1 产生的成核频率（即冰核/细胞的数量）比冰成核丁香假单胞菌 31 号悬浮液低，特别是在高于零下 5 度的温度下。 C.菌株KUIN-1-悬浮液的成核频率与在-5℃下冰成核的草本欧文氏菌No.26获得的成核频率相似。 C.
An ice-nucleating bacterium strain KUIN-1, was isolated from the leaves of field beans (Phaseolus vulgaris L.) Strain KUIN-1 was identified as Pseudomonas fluorescens from its taxonomical characteristics. Ice-nucleating activity was obtained when strain KUIN-1 was cultured aerobically in a medium containing Koser citrate broth (pH 7.0) for 24hr at 18.degree. C. The ice-nucleating activity did not appear until the bacterial cell concentration reached 107 to 108/ml. Nucleation at - 3.0.degree. C was detected in suspension (1.8 .times. 109 cells/ml) of cells that had been grown on the medium containing Koser citrate broth. Strain KUIN-1 produced a lower nucleation frequency (i.e. the number of ice nuclei/cell) than did ice-nucleating Pseudomonas syringae No. 31 suspension, particularly at temperatures above - 5.degree. C. The nucleation frequency of strain KUIN-1-suspensions was similar to that obtained for an ice-nucleating Erwinia herbicola No. 26 at -5.degree. C.