Photoacoustic detection of metastatic melanoma cells in the human circulatory system
Photoacoustic detection of metastatic melanoma cells in the human circulatory system
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DOI：
10.1364/ol.31.002998
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发表时间：
2006-10-15
期刊：
影响因子：
3.6
通讯作者：
Lisle, Allison E.
中科院分区：
文献类型：
--
作者：
Weight, Ryan M.;Viator, John A.;Lisle, Allison E.
在患有各种类型和分期的癌症患者中检测播散性肿瘤细胞可作为一种预警系统，提醒医生疾病的转移扩散或复发。早期检测到此类细胞可导致对疾病的预防性治疗，而晚期检测可作为化疗有效性的一个指标。揭示播散性肿瘤细胞的预后价值迫切需要一种高效、准确的转移性细胞筛选方法。我们提出一种基于黑色素瘤热弹性特性的转移性循环肿瘤细胞检测系统。该方法采用光声激发，并结合一个能够在体外确定循环系统内播散细胞存在的检测系统。由组织模型和人黑色素瘤细胞系组成的检测试验得出的检测阈值约为10个单个细胞，从而验证了所提出机制的有效性。结果暗示有可能对健康和转移性患者的简单血液样本进行检测，以确定是否存在癌性黑色素瘤，为常规癌症筛查提供一种前所未有的方法。（c）2006美国光学学会
Detection of disseminating tumor cells among patients suffering from various types and stages of cancer can function as an early warning system, alerting the physician of the metastatic spread or recurrence of the disease. Early detection of such cells can result in preventative treatment of the disease, while late stage detection can serve as an indicator of the effectiveness of chemotherapeutics. The prognostic value of exposing disseminating tumor cells poses an urgent need for an efficient, accurate screening method for metastatic cells. We propose a system for the detection of metastatic circulating tumor cells based on the thermoelastic properties of melanoma. The method employs photoacoustic excitation coupled with a detection system capable of determining the presence of disseminating cells within the circulatory system in vitro. Detection trials consisting of tissue phantoms and a human melanoma cell line resulted in a detection threshold of the order of ten individual cells, thus validating the effectiveness of the proposed mechanism. Results imply the potential to assay simple blood draws, from healthy and metastatic patients, for the presence of cancerous melanoma providing an unprecedented method for routine cancer screening. (c) 2006 Optical Society of America.