Time-resolved absorption studies of native etiolated oat phytochrome

Time-resolved absorption studies of native etiolated oat phytochrome
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天然黄化燕麦光敏色素的时间分辨吸收研究

DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
D. Kliger
D. Kliger
中科院分区：
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文献类型：
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作者：
Chian;D. Farrens;S. C. Bjorling;P. Song;D. Kliger

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在10 ° C条件下，采用激光光解实验研究了燕麦黄化苗中光敏色素的光转化动力学。由红色吸收形式（Pr）到远红色吸收形式（Pfr）的光转化由7-ns（fwhm）的激光脉冲在638 nm处引发。用门控光学多道分析系统记录了光解后100 ns ~ 800 ms的紫外和可见光区的瞬态吸收差谱。采用奇异值分解法进行全局拟合，同时分析了光解后不同时间的差异光谱
The phototransformation kinetics of native oat phytochrome from etiolated seedling shoots of Avena sativa was investigated by laser photolysis experiments at 10 o C. The phototransformation from the red-absorbing form (Pr) to the far-red-absorbing form (Pfr) was initiated by 7-ns (fwhm) laser pulses at 638 nm. The transient absorption difference spectra in the UV and visible regions were recorded from 100 ns to 800 ms after photolysis by a gated optical multichannel analyzer system. A global fitting procedure employing a singular value decomposition method was used to simultaneously analyze the difference spectra at various times after photolysis