SKELETAL-MUSCLE GLYCOLYSIS, OXIDATION, AND STORAGE OF AN ORAL GLUCOSE-LOAD
SKELETAL-MUSCLE GLYCOLYSIS, OXIDATION, AND STORAGE OF AN ORAL GLUCOSE-LOAD
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DOI：
10.1172/jci113489
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发表时间：
1988-05-01
影响因子：
15.9
通讯作者：
GERICH, J
中科院分区：
文献类型：
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作者：
KELLEY, D;MITRAKOU, A;GERICH, J
尽管肌肉被认为是餐后葡萄糖处理的最重要部位，但肌肉吸收的口服葡萄糖的代谢命运仍不清楚。因此，我们采用双同位素技术（静脉注射，[6-3H]-葡萄糖；口服，[1-14C]葡萄糖）、间接量热法和前臂平衡测量九名正常志愿者的葡萄糖、乳酸、丙氨酸、丙酮酸、O2和CO2，以确定肌肉生糖、糖酵解和氧化途径在处理口服葡萄糖负荷中的相对重要性。在葡萄糖摄入（1 g/kg）后5小时内，37.+-.3%（24.9.+-.2.3 g）的负载被氧化，63.+-.3%（42.8.+-.2.7 g）被储存。至少 29% (19.4.+-.1.3 g) 被内脏组织吸收。肌肉吸收了 26% (17.9.+-.2.9 g) 的口服葡萄糖，同时脂肪氧化减少了 50%。肌肉吸收的口服葡萄糖的 15% (2.5.+-.0.9 g) 以乳酸盐、丙氨酸或丙酮酸盐的形式释放； 50% (8.9.+-.1.4 g) 被氧化，35% (6.4.+-.2.3 g) 可用于储存。我们得出的结论是，肌肉和内脏组织占据了相当比例的口服葡萄糖负荷，并且氧化是肌肉吸收葡萄糖的主要命运，而肌肉中的储存占口服葡萄糖负荷的<10%。因此，与普遍观点相反，肌肉既不是主要的储存部位，也不是口服葡萄糖负荷的主要处理部位。
Although muscle is considered to be the most important site for postprandial glucose disposal, the metabolic fate of oral glucose taken up by muscle remains unclear. We, therefore, employed the dual isotope technique (intravenous, [6-3H]-glucose; oral, [1-14C]glucose), indirect calorimetry, and forearm balance measurements of glucose, lactate, alanine, pyruvate, O2, and CO2 in nine normal volunteers to determine the relative importance of muscle glycogenic, glycolytic, and oxidative pathways in disposal of an oral glucose load. During the 5 h after glucose ingestion (1 g/kg), 37.+-.3% (24.9.+-.2.3 g) of the load was oxidized and 63.+-.3% (42.8.+-.2.7 g) was stored. At least 29% (19.4.+-.1.3 g) was taken up by splanchnic tissues. Muscle took up 26% (17.9.+-.2.9 g) of the oral glucose coincident with a 50% reduction in its oxidation of fat. 15% of the oral glucose taken up by muscle (2.5.+-.0.9 g) was released as lactate, alanine, or pyruvate; 50% (8.9.+-.1.4 g) was oxidized, and 35% (6.4.+-.2.3 g) was available for storage. We conclude that muscle and splanchnic tissues take up a comparable percentage of an oral glucose load and that oxidation is the predominant fate of glucose take up by muscle, with storage in muscle accounting for < 10% of the oral load. Thus, contrary to the prevailing view, muscle is neither the major site of storage nor the predominant site of disposal of an oral glucose load.