WRKY transcription factors actively respond to Fusarium oxysporum in Lilium regale Wilson
WRKY transcription factors actively respond to Fusarium oxysporum in Lilium regale Wilson
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DOI：
10.1094/phyto-10-20-0480-r
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Diqiu Liu
中科院分区：
文献类型：
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作者：
Shan Li;Guanze Liu;Limei Pu;Xuyan Liu;Zie Wang;Qin Zhao;Hongjun Chen;Feng Ge;Diqiu Liu
The WRKY transcription factors (TFs) form a plant-specific superfamily important for regulating plant development, stress responses, and hormone signal transduction. In this study, many WRKY genes (LrWRKY1–35) were identified in Lilium regale Wilson, which is a wild lily species highly resistant to Fusarium wilt. These WRKY genes were divided into three classes (I–III) based on a.phylogenetic analysis. The Class II WRKY TFs were further divided into five subclasses (IIa, IIb, IIc, IId, and IIe). Moreover, the gene expression patterns based on a quantitative real-time PCR analysis revealed the WRKY genes were differentially expressed in the L. regale roots, stems, leaves, and flowers. Additionally, the expression of the WRKY genes was affected by an infection by Fusarium oxysporum as well as by salicylic acid, methyl jasmonate, ethylene, and hydrogen peroxide treatments. Moreover, the LrWRKY1 protein was localized to the nucleus of onion epidermal cells. The recombinant LrWRKY1 protein purified from Escherichia coli bound specifically to DNA fragments containing the W-box sequence, and a yeast one-hybrid assay indicated that LrWRKY1 can activate transcription. Furthermore, the overexpression of LrWRKY1 in tobacco increased the resistance of the transgenic plants to F. oxysporum. In conclusion, this study 58 provides valuable information regarding the expression and functional characteristics of the L. regale WRKY genes. The results presented herein may further clarify the transcriptional regulatory mechanism underlying L. regale responses to F. oxysporum infections.
