H-FABP and LEPR gene expression profile in skeletal muscles and liver during ontogenesis in various breeds of pigs
H-FABP and LEPR gene expression profile in skeletal muscles and liver during ontogenesis in various breeds of pigs
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DOI：
10.1016/j.domaniend.2010.10.001
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发表时间：
2011-04-01
影响因子：
2.1
通讯作者：
Oczkowicz, M.
中科院分区：
文献类型：
--
作者：
Tyra, M.;Ropka-Molik, K.;Oczkowicz, M.
编码H-FABP(心脏酸结合蛋白)和LEPR(瘦素受体)的基因被认为是脂肪代谢的候选基因，从而影响猪的脂肪沉积。本研究的目的是研究H-FABP和LEPR在不同日龄猪的骨骼肌(背最长肌、半膜肌)和肝脏中的转录水平。这些实验是在波兰饲养的5个流行品种的猪身上进行的，这些猪表现出不同程度的脂肪组织。此外，我们还检测了H-FABP和LEPR基因(H-FABP的HinfI、HpaII和HaeII以及LEPR的HpaII)对这些基因表达丰度的影响。我们证实了品种(P<；.001)、组织类型(LEPR P<；.001；H-FABP P<；.01)和动物年龄(P<；.05)对这两个基因的mRNA转录丰度的影响具有统计学意义。在所有品种中，瘦素受体基因的表达随着年龄的增长在肌肉组织中显著增加(P<；.01)，而在肝脏组织中没有观察到这种关系。然而，H-FABP基因在肌肉中的表达不随年龄或品种的变化而变化，而在肝脏中的表达水平在仔猪(60d和90d)中较高。综上所述，H-FABP和LEPR基因与猪脂肪组织的发育和功能密切相关。(C)2011 Elsevier Inc.保留所有权利。
The genes coding for H-FABP (heart acid-binding protein) and LEPR (leptin receptor) are considered to be candidates for lipid metabolism and thus affect fat deposition in pigs. The aim of our study was to assess the amount of H-FABP and LEPR transcript in the skeletal muscles (m. longissimus dorsi, m. semimembranosus) and liver of pigs of various ages. The experiments were carried out on 5 popular breeds of swine raised in Poland which exhibit different levels of fat tissue. Furthermore, we examined the effect of H-FABP and LEPR genotypes (HinfI, HpaII, and HaeII for H-FABP and HpaII for LEPR) on the expression abundance of these genes. We confirmed a statistically significant relationship between the breed (P < .001), type of tissue (LEPR P < .001; H-FABP P < .01), and age of the animal (P < .05) on the abundance of mRNA transcript of both genes. In all breeds, the expression of the leptin receptor gene increased significantly (P < .01) with age in muscle tissue, whereas this relationship was not observed in liver tissue. However, the expression of the H-FABP gene in muscles did not change with age or breed, although in the liver expression levels were high in young (60 and 90 d) pigs. In conclusion, H-FABP and LEPR genes are strongly related to the development and function of fat tissue in pigs. (C) 2011 Elsevier Inc. All rights reserved.