RADIOSENSITIVITY OF MULTICELLULAR TUMOR SPHEROIDS OBTAINED FROM HUMAN OVARIAN CANCERS
RADIOSENSITIVITY OF MULTICELLULAR TUMOR SPHEROIDS OBTAINED FROM HUMAN OVARIAN CANCERS
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DOI：
10.1016/0959-8049(94)00377-h
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发表时间：
1995-01-01
影响因子：
8.4
通讯作者：
BEY, P
中科院分区：
文献类型：
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作者：
GRIFFON, G;MARCHAL, C;BEY, P
使用多细胞肿瘤球体（MTS）三维模型，体外测定了从积液或实体瘤中获得的免疫学表征（KL（1）、抗波形蛋白和OC 125）人卵巢癌细胞的放射反应性。MTS倍增时间存在较大的样本间变异性（1.0-8.5天），以及诱导MTS个体体积减少50%的剂量（ID 50）（0.56-9.15戈伊），或在总体MTS数（SCD 50）中（1.9-15.7戈伊）和2戈伊照射后残留/初始MTS个体体积比（RSV（2））（10-88%）。倍增时间、DNA倍体和S期细胞比例与ID 50无相关性。发现定义的新参数（RSV（2）和ID 50）与公认的局部对照参数SCD 50之间存在显著相关性。这些参数证明了它们对研究从人卵巢肿瘤活检制备的MTS的放射敏感性的有用性。
The radioresponsiveness of immunologically characterised (KL(1), antivimentin and OC125) human ovarian carcinoma cells, obtained from effusions or solid tumours, was assayed in vitro using the multicellular tumour spheroids (MTS) three-dimensional model. Great interspecimen variabilities were observed in MTS doubling time (1.0-8.5 days), as well as in the doses inducing a 50% decrease in the MTS individual volume (ID50) (0.56-9.15 Gy), or in the overall population MTS number (SCD50) (1.9-15.7 Gy) and the residual/initial MTS individual volume ratio after 2 Gy irradiation (RSV(2)) (10-88%). The doubling time, DNA-ploidy and S-phase fraction did not correlate with the ID50. Significant correlations were found between the new parameters defined (RSV(2) and ID50) and the SCD50, a well-accepted local control parameter. These parameters demonstrated their usefulness for studying the radiosensitivity of MTS prepared from human ovarian tumour biopsies.