A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis.
A putative RNA-binding protein positively regulates salicylic acid-mediated immunity in Arabidopsis.
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DOI：
10.1094/mpmi-05-10-0106
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发表时间：
2010-11
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影响因子：
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通讯作者：
Yiping Qi;K. Tsuda;Anna Joe;Masanao Sato;L. Nguyen;J. Glazebrook;J. Alfano;J. Cohen;F. Katagiri
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文献类型：
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作者：
Yiping Qi;K. Tsuda;Anna Joe;Masanao Sato;L. Nguyen;J. Glazebrook;J. Alfano;J. Cohen;F. Katagiri
RNA结合蛋白（RBP）可以在转录和转录后水平调控基因表达。植物通过基因表达的快速重编程响应病原体感染。然而，对植物RBP在植物免疫中的作用知之甚少。在这里，我们描述了参与的RBP，拟南芥RNA结合蛋白防御相关1（AtRBP-DR1; At4g03110），在抗病原体假单胞菌pv.番茄DC3000。AtRBP-DR1功能缺失突变体对P.番茄DC3000。AtRBP-DR1的过表达导致对P.番茄DC3000株系和矮化。该过敏反应由P.番茄DC3000 avrRpt 2在Atrbp-dr1突变体中受损，在AtRBP-DR1过表达系中在早期时间点增强。AtRBP-DR1过表达株系表现出更高的SID2和PR1 mRNA水平，这是水杨酸（SA）诱导的，以及成熟叶片中的自发细胞死亡。与这些观察结果一致，SA水平在Atrbp-dr1突变体中低，但在过表达系中高。SA相关表型在过表达系中完全依赖于SID2。因此，AtRBP-DR1是SA介导的免疫的正调节因子，可能在转录后水平作用于SA信号相关基因。
RNA-binding proteins (RBP) can control gene expression at both transcriptional and post-transcriptional levels. Plants respond to pathogen infection with rapid reprogramming of gene expression. However, little is known about how plant RBP function in plant immunity. Here, we describe the involvement of an RBP, Arabidopsis thaliana RNA-binding protein-defense related 1 (AtRBP-DR1; At4g03110), in resistance to the pathogen Pseudomonas syringae pv. tomato DC3000. AtRBP-DR1 loss-of-function mutants showed enhanced susceptibility to P. syringae pv. tomato DC3000. Overexpression of AtRBP-DR1 led to enhanced resistance to P. syringae pv. tomato DC3000 strains and dwarfism. The hypersensitive response triggered by P. syringae pv. tomato DC3000 avrRpt2 was compromised in the Atrbp-dr1 mutant and enhanced in the AtRBP-DR1 overexpression line at early time points. AtRBP-DR1 overexpression lines showed higher mRNA levels of SID2 and PR1, which are salicylic acid (SA) inducible, as well as spontaneous cell death in mature leaves. Consistent with these observations, the SA level was low in the Atrbp-dr1 mutant but high in the overexpression line. The SA-related phenotype in the overexpression line was fully dependent on SID2. Thus, AtRBP-DR1 is a positive regulator of SA-mediated immunity, possibly acting on SA signaling-related genes at a post-transcriptional level.